Menaquinone-7 regulates gene expression in osteoblastic MC3T3E1 cells

Katsuyama,Hironobu; Saijoh,Kiyofumi; Otsuki,Takemi; Tomita,Masafumi; Fukunaga,Masao; Sunami,Shigeo

doi:10.3892/ijmm.19.2.279

February 2007 Volume 19 Issue 2

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February 2007 Volume 19 Issue 2

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Article

Menaquinone-7 regulates gene expression in osteoblastic MC3T3E1 cells

Authors:
- Hironobu Katsuyama
- Kiyofumi Saijoh
- Takemi Otsuki
- Masafumi Tomita
- Masao Fukunaga
- Shigeo Sunami
View Affiliations / Copyright

Affiliations: Department of Public Health, Kawasaki Medical School, Kurashiki 701-0192, Japan. katsu@med.kawasaki-m.ac.jp
Pages: 279-284
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Published online on: February 1, 2007

https://doi.org/10.3892/ijmm.19.2.279
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Abstract

Previous study has shown that the vitamin K2 analog menaquinone-7 (MK-7) induces expression of the osteoblast-specific genes osteocalcin, osteoprotegerin, receptor activator of NFκB, and its ligand. Since MK-7 may also regulate osteoblast cell function, we examined the expression of osteoblast genes regulated by MK-7 administration. Differences between gene expression in control and MK-7-administered MC3T3E1 cells were analyzed using the suppression subtractive hybridization method. After 24 h of MK-7 administration, genes upregulated by MK-7 included tenascin C and BMP2. Genes downregulated by MK-7 administration included biglycan and butyrophilin. Real-time PCR showed a marked increase in tenascin C. When the protein level was examined using Western blot analysis, tenascin C was higher in MK-7-administered cells than in control cells. These results indicated that MK-7 affected the cellular function of osteoblastic MC3T3E1 cells. Considering BMP2 mRNA expression was higher in MK-7-administered cells than in control cells, the effect of MK-7 administration on the signal transduction system was examined. Western blot analysis showed that cells administered MK-7 displayed a higher phosphorylated Smad1 level than control cells. Because MC3T3E1 cells have a nuclear binding receptor for MK-7, this result might indicate an indirect effect of MK-7 through BMP2 production.

Journals

International Journal of Molecular Medicine

International Journal of Oncology

Molecular Medicine Reports

Oncology Reports

Experimental and Therapeutic Medicine

Oncology Letters

Biomedical Reports

Molecular and Clinical Oncology

World Academy of Sciences Journal

International Journal of Functional Nutrition

International Journal of Epigenetics

Medicine International

Menaquinone-7 regulates gene expression in osteoblastic MC3T3E1 cells

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Abstract

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