REIC/Dkk-3 as a potential gene therapeutic agent against human testicular cancer

  • Authors:
    • Ryuta Tanimoto
    • Fernando Abarzua
    • Masakiyo Sakaguchi
    • Mikiro Takaishi
    • Yasutomo Nasu
    • Hiromi Kumon
    • Nam-Ho Huh
  • View Affiliations

  • Published online on: March 1, 2007     https://doi.org/10.3892/ijmm.19.3.363
  • Pages: 363-368
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Abstract

Human testicular cancer is very sensitive to chemotherapy and radiation therapy and is regarded as a curable cancer. The cancer prevails in the young reproductive generation and testicular dysfunction is often observed as a side effect, remaining a serious challenge. In the present study, we examined the potential utility of REIC/Dkk-3-based gene therapy against human testicular cancer. Expression of REIC/Dkk-3 was reduced in all of the human seminoma and non-seminomatous germ cell tumor tissues. Overexpression of REIC/Dkk-3 using an adenovirus vector (Ad-REIC) induced apoptosis in a testicular germ cell cancer cell line NCCIT but not in normal human fibroblasts. c-Jun terminal kinase (JNK) was activated by Ad-REIC and the induction of apoptosis was abrogated by a JNK inhibitor. A single intratumoral injection of Ad-REIC markedly inhibited the tumorigenic growth of NCCIT cells in nude mice. These results indicate that Ad-REIC may lead to developing less insulting and non-genotoxic therapeutic measures against human testicular cancer.

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March 2007
Volume 19 Issue 3

Print ISSN: 1107-3756
Online ISSN:1791-244X

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Spandidos Publications style
Tanimoto R, Abarzua F, Sakaguchi M, Takaishi M, Nasu Y, Kumon H and Huh N: REIC/Dkk-3 as a potential gene therapeutic agent against human testicular cancer. Int J Mol Med 19: 363-368, 2007.
APA
Tanimoto, R., Abarzua, F., Sakaguchi, M., Takaishi, M., Nasu, Y., Kumon, H., & Huh, N. (2007). REIC/Dkk-3 as a potential gene therapeutic agent against human testicular cancer. International Journal of Molecular Medicine, 19, 363-368. https://doi.org/10.3892/ijmm.19.3.363
MLA
Tanimoto, R., Abarzua, F., Sakaguchi, M., Takaishi, M., Nasu, Y., Kumon, H., Huh, N."REIC/Dkk-3 as a potential gene therapeutic agent against human testicular cancer". International Journal of Molecular Medicine 19.3 (2007): 363-368.
Chicago
Tanimoto, R., Abarzua, F., Sakaguchi, M., Takaishi, M., Nasu, Y., Kumon, H., Huh, N."REIC/Dkk-3 as a potential gene therapeutic agent against human testicular cancer". International Journal of Molecular Medicine 19, no. 3 (2007): 363-368. https://doi.org/10.3892/ijmm.19.3.363