Dose-dependent expression changes of early response genes to ionizing radiation in human lymphoblastoid cells

  • Authors:
    • Xian-Hui Long
    • Zeng-Qiang Zhao
    • Xing-Peng He
    • Hui-Ping Wang
    • Qin-Zhi Xu
    • Jing An
    • Bei Bai
    • Jian-Li Sui
    • Ping-Kun Zhou
  • View Affiliations

  • Published online on: Sunday, April 1, 2007
  • Pages:607-615 DOI: 10.3892/ijmm.19.4.607
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Abstract

The sensitivity of cancer cells as well as normal cells in response to ionizing radiation (IR) is believed to be associated with the early inducible expression of specific genes. Using cDNA microarray technology, here we explored and compared the global transcriptional changes in human lymphoblastoid AHH-1 cells irradiated with 0.05-, 0.2-, 0.5-, 2.0- and 10-Gy doses of γ-rays 4 h after exposure. A dose as low as 0.05 Gy was efficient in inducing a transcriptional response including the up-regulation of 25 genes, some of which are involved in signal transduction pathways, e.g. BMPR2, GPR124, MAPK8IP2 and AGGF1, and the down-regulation of 18 genes. Expression of some genes was altered only at a specific dose. Most importantly, we discovered a number of radiation-response genes, e.g. DNA repair gene XPC, tumor protein p53 inducible protein 3 gene (TP53I3), immediate early response 5 gene, whose transcriptional levels were increased or depressed by IR in a dose-dependent trend within the dose range 0.05-10 Gy. The dose-dependent induced expression of TP53I3 and XPC was confirmed by Northern blot analyses. Using quantitative real-time PCR, we further confirmed that XPC gene induction was dose dependent as well as time dependent, reaching a peak 4 h post-2 Gy and 10 h post-0.05 Gy. The maximum induced expression level of the XPC gene was higher after 2 Gy (3.2-fold) than 0.05 Gy (1.93-fold). The identification of these radiation-inducible genes, especially those exhibiting a dose-dependent response, not only expands our knowledge of the mechanisms underlying the diverse biological effects induced by IR, but provides candidates for developing novel biomarkers of radiation injury.

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April 2007
Volume 19 Issue 4

Print ISSN: 1107-3756
Online ISSN:1791-244X

2015 Impact Factor: 2.348
Ranked #22/123 Medicine Research and Experimental
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APA
Long, X., Zhao, Z., He, X., Wang, H., Xu, Q., An, J. ... Zhou, P. (2007). Dose-dependent expression changes of early response genes to ionizing radiation in human lymphoblastoid cells. International Journal of Molecular Medicine, 19, 607-615. http://dx.doi.org/10.3892/ijmm.19.4.607
MLA
Long, X., Zhao, Z., He, X., Wang, H., Xu, Q., An, J., Bai, B., Sui, J., Zhou, P."Dose-dependent expression changes of early response genes to ionizing radiation in human lymphoblastoid cells". International Journal of Molecular Medicine 19.4 (2007): 607-615.
Chicago
Long, X., Zhao, Z., He, X., Wang, H., Xu, Q., An, J., Bai, B., Sui, J., Zhou, P."Dose-dependent expression changes of early response genes to ionizing radiation in human lymphoblastoid cells". International Journal of Molecular Medicine 19, no. 4 (2007): 607-615. http://dx.doi.org/10.3892/ijmm.19.4.607