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International Journal of Molecular Medicine
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Print ISSN: 1107-3756 Online ISSN: 1791-244X
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June 2007 Volume 19 Issue 6

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International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

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Medicine International

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Article

Integrated in silico and biological validation of the blocking effect of Cot-1 DNA on Microarray-CGH

  • Authors:
    • Seung-Hui Kang
    • Chan Hee Park
    • Hei Cheul Jeung
    • Ki-Yeol Kim
    • Sun Young Rha
    • Hyun Cheol Chung

  • View Affiliations / Copyright

    Affiliations: Cancer Metastasis Research Center, Yonsei Cancer Center, Yonsei University College of Medicine, Seoul 120-752, Korea
  • Pages: 901-908
    |
    Published online on: June 1, 2007
       https://doi.org/10.3892/ijmm.19.6.901
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Abstract

In array-CGH, various factors may act as variables influencing the result of experiments. Among them, Cot-1 DNA, which has been used as a repetitive sequence-blocking agent, may become an artifact-inducing factor in BAC array-CGH. To identify the effect of Cot-1 DNA on Microarray-CGH experiments, Cot-1 DNA was labeled directly and Microarray-CGH experiments were performed. The results confirmed that probes which hybridized more completely with Cot-1 DNA had a higher sequence similarity to the Alu element. Further, in the sex-mismatched Microarray-CGH experiments, the variation and intensity in the fluorescent signal were reduced in the high intensity probe group in which probes were better hybridized with Cot-1 DNA. Otherwise, those of the low intensity probe group showed no alterations regardless of Cot-1 DNA. These results confirmed by in silico methods that Cot-1 DNA could block repetitive sequences in gDNA and probes. In addition, it was confirmed biologically that the blocking effect of Cot-1 DNA could be presented via its repetitive sequences, especially Alu elements. Thus, in contrast to BAC-array CGH, the use of Cot-1 DNA is advantageous in controlling experimental variation in Microarray-CGH.

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Copy and paste a formatted citation
Spandidos Publications style
Kang S, Park CH, Jeung HC, Kim K, Rha SY and Chung HC: Integrated in silico and biological validation of the blocking effect of Cot-1 DNA on Microarray-CGH. Int J Mol Med 19: 901-908, 2007.
APA
Kang, S., Park, C.H., Jeung, H.C., Kim, K., Rha, S.Y., & Chung, H.C. (2007). Integrated in silico and biological validation of the blocking effect of Cot-1 DNA on Microarray-CGH. International Journal of Molecular Medicine, 19, 901-908. https://doi.org/10.3892/ijmm.19.6.901
MLA
Kang, S., Park, C. H., Jeung, H. C., Kim, K., Rha, S. Y., Chung, H. C."Integrated in silico and biological validation of the blocking effect of Cot-1 DNA on Microarray-CGH". International Journal of Molecular Medicine 19.6 (2007): 901-908.
Chicago
Kang, S., Park, C. H., Jeung, H. C., Kim, K., Rha, S. Y., Chung, H. C."Integrated in silico and biological validation of the blocking effect of Cot-1 DNA on Microarray-CGH". International Journal of Molecular Medicine 19, no. 6 (2007): 901-908. https://doi.org/10.3892/ijmm.19.6.901
Copy and paste a formatted citation
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Spandidos Publications style
Kang S, Park CH, Jeung HC, Kim K, Rha SY and Chung HC: Integrated in silico and biological validation of the blocking effect of Cot-1 DNA on Microarray-CGH. Int J Mol Med 19: 901-908, 2007.
APA
Kang, S., Park, C.H., Jeung, H.C., Kim, K., Rha, S.Y., & Chung, H.C. (2007). Integrated in silico and biological validation of the blocking effect of Cot-1 DNA on Microarray-CGH. International Journal of Molecular Medicine, 19, 901-908. https://doi.org/10.3892/ijmm.19.6.901
MLA
Kang, S., Park, C. H., Jeung, H. C., Kim, K., Rha, S. Y., Chung, H. C."Integrated in silico and biological validation of the blocking effect of Cot-1 DNA on Microarray-CGH". International Journal of Molecular Medicine 19.6 (2007): 901-908.
Chicago
Kang, S., Park, C. H., Jeung, H. C., Kim, K., Rha, S. Y., Chung, H. C."Integrated in silico and biological validation of the blocking effect of Cot-1 DNA on Microarray-CGH". International Journal of Molecular Medicine 19, no. 6 (2007): 901-908. https://doi.org/10.3892/ijmm.19.6.901
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