Cytotoxicity of Fas ligand against lymphoma cells with radiation-induced Fas antigen.

  • Authors:
    • Y Ogawa
    • A Nishioka
    • I Kubonishi
    • T Inomata
    • S Yoshida
    • S Kataoka
  • View Affiliations

  • Published online on: October 1, 1998     https://doi.org/10.3892/ijmm.2.4.435
  • Pages: 435-441
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Abstract

Fas antigen, also termed APO-1 or CD95, is a transmembrane protein and a member of the tumor necrosis factor receptor/nerve growth factor receptor superfamily which mediates apoptosis upon oligomerization. The Fas/Fas ligand system is considered to be a key regulator of apoptosis. Recently, we have demonstrated that Fas antigen expression is induced by low-dose irradiation of some types of lymphomas, and we also demonstrated that irradiation-induced Fas antigen expression increased with the passage of time until peaking at 48 h after irradiation in CML-C1, CML-C2, DL-40, and DL-95 cell lines. In this study, we also examined the potential cytotoxicity of Fas ligand peptide against several types of lymphoma/leukemia cell lines that showed induction of Fas antigen expression under irradiation. Flow cytometry analysis was performed at 6, 24 and 48 h after irradiation. Samples (1 x10(6) cells/ml) from irradiated and non-irradiated cells of each cell line were incubated with or without 5 microg/ml of Fas ligand peptide for 2 h at 37 degrees C in a humidified atmosphere of 5% carbon dioxide (CO2) in air. The killing effect of Fas ligand against cell lines of CML-C1, DL-40, and DL-95 were clearly identified as the percentage of cells with Fas antigen expression induced by irradiation. Concerning HD-70 cell line, for which soluble Fas antigen has been identified, the killing effects were clearly observed in samples pre-treated with PBS washings. To our knowledge, this is the first report describing a possible application of the Fas/Fas ligand system in treatment of certain types of malignancies in which Fas antigen is inducible by irradiation.

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Oct 1998
Volume 2 Issue 4

Print ISSN: 1107-3756
Online ISSN:1791-244X

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Spandidos Publications style
Ogawa Y, Nishioka A, Kubonishi I, Inomata T, Yoshida S and Kataoka S: Cytotoxicity of Fas ligand against lymphoma cells with radiation-induced Fas antigen.. Int J Mol Med 2: 435-441, 1998.
APA
Ogawa, Y., Nishioka, A., Kubonishi, I., Inomata, T., Yoshida, S., & Kataoka, S. (1998). Cytotoxicity of Fas ligand against lymphoma cells with radiation-induced Fas antigen.. International Journal of Molecular Medicine, 2, 435-441. https://doi.org/10.3892/ijmm.2.4.435
MLA
Ogawa, Y., Nishioka, A., Kubonishi, I., Inomata, T., Yoshida, S., Kataoka, S."Cytotoxicity of Fas ligand against lymphoma cells with radiation-induced Fas antigen.". International Journal of Molecular Medicine 2.4 (1998): 435-441.
Chicago
Ogawa, Y., Nishioka, A., Kubonishi, I., Inomata, T., Yoshida, S., Kataoka, S."Cytotoxicity of Fas ligand against lymphoma cells with radiation-induced Fas antigen.". International Journal of Molecular Medicine 2, no. 4 (1998): 435-441. https://doi.org/10.3892/ijmm.2.4.435