Setting up of an original computer-assisted methodology to characterize in vitro drug-induced anti-angiogenic effects.

Farinelle,S; Dehauwer,C; Darro,F; Decaestecker,C; Fontaine,J; Pasteels,J L; Van Ham,P; Atassi,G; Kiss,R

doi:10.3892/ijmm.2.5.545

Nov 1998 Volume 2 Issue 5

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Nov 1998 Volume 2 Issue 5

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Article

Setting up of an original computer-assisted methodology to characterize in vitro drug-induced anti-angiogenic effects.

Authors:
- S Farinelle
- C Dehauwer
- F Darro
- C Decaestecker
- J Fontaine
- J L Pasteels
- P Van Ham
- G Atassi
- R Kiss
View Affiliations / Copyright

Affiliations: Laboratoire d'Histologie, Faculté de Medecine, Université Libre de Bruxelles, Brussels, Belgium.
Pages: 545-598
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Published online on: November 1, 1998

https://doi.org/10.3892/ijmm.2.5.545
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Abstract

The development of angiogenesis within a tumor brings on a sequence of extremely complex molecular events. We have developed a methodology which enables a wide set of biological parameters to be quantitatively determined in the field of anti-angiogenesis pharmacology. This methodology which includes a video cell tracking device, is unique because it offers the possibility of evaluating the specific influence of a given compound with potential anti-angiogenic properties on cell cycle kinetics, cell death, global cell line growth, and cell motility. We chose TNP-470, a synthetic analogue of fumagilin, to test our methodology on HUVEC cell lines taken from various human umbilical cord veins. The experiments carried out with TNP-470 did not confirm all the data reported in the literature. Our results show that i) TNP-470 could be considered as a cytotoxic agent; ii) this compound had an apparently marginal cytostatic effect; and iii) it did not increase the apoptosis level. Our methodology also revealed that the HUVEC cell lines are very heterogeneous in terms of different biological parameters. This highlights the problem of the reproductibility of the result.

Journals

International Journal of Molecular Medicine

International Journal of Oncology

Molecular Medicine Reports

Oncology Reports

Experimental and Therapeutic Medicine

Oncology Letters

Biomedical Reports

Molecular and Clinical Oncology

World Academy of Sciences Journal

International Journal of Functional Nutrition

International Journal of Epigenetics

Medicine International

Setting up of an original computer-assisted methodology to characterize in vitro drug-induced anti-angiogenic effects.

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Abstract

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