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International Journal of Molecular Medicine
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Print ISSN: 1107-3756 Online ISSN: 1791-244X
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July 2007 Volume 20 Issue 1

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Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

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July 2007 Volume 20 Issue 1

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Article

Ultrastructural demonstration of hepatitis B virus production in a mouse model produced by hydrodynamic transfection

  • Authors:
    • Masayoshi Konishi
    • Hideaki Tanaka
    • Masahiko Kaito
    • Naoki Fujita
    • Motoh Iwasa
    • Yoshinao Kobayashi
    • Yukihiko Adachi
    • Shozo Watanabe

  • View Affiliations / Copyright

    Affiliations: Center for Physical and Mental Health, Mie University, Mie 514-8507, Japan. mkonishi@ab.mie-u.ac.jp
  • Pages: 31-36
    |
    Published online on: July 1, 2007
       https://doi.org/10.3892/ijmm.20.1.31
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Abstract

A mouse model of hepatitis B virus (HBV) infection produced by hydrodynamic injection of HBV DNA has been recently established. However, the ultrastructural demonstration of HBV particles in this mouse model has not as yet been reported. In our study, plasmid DNA containing wild-type HBV DNA was rapidly injected into 8-week-old female SCID mice via the tail vein. Serum levels of HBsAg were measured by ELISA kit. Intrahepatic HBV protein expression was detected by immunohistochemistry of HBcAg. Ultrastructural study of the serum samples was performed by transmission electron microscopy and immunogold electron microscopy. Serum HBsAg and intrahepatic HBcAg were detected in HBV DNA-injected mice for at least 14 days. Spherical and filamentous particles 22 nm in diameter and double-shelled Dane-like particles 42 nm in diameter were detected in the sera of mice. The ultrastructural features of these particles were identical to HBV particles observed in serum from chronic hepatitis B patients. These particles were confirmed to be HBV particles by immunogold electron microscopy. We conclude that our present HBV mouse model using hydrodynamic transfection of HBV DNA is appropriate for production of HBV virions including Dane particles. This mouse model may be useful for screening in vivo the efficacy of antiviral drugs.

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Copy and paste a formatted citation
Spandidos Publications style
Konishi M, Tanaka H, Kaito M, Fujita N, Iwasa M, Kobayashi Y, Adachi Y and Watanabe S: Ultrastructural demonstration of hepatitis B virus production in a mouse model produced by hydrodynamic transfection. Int J Mol Med 20: 31-36, 2007.
APA
Konishi, M., Tanaka, H., Kaito, M., Fujita, N., Iwasa, M., Kobayashi, Y. ... Watanabe, S. (2007). Ultrastructural demonstration of hepatitis B virus production in a mouse model produced by hydrodynamic transfection. International Journal of Molecular Medicine, 20, 31-36. https://doi.org/10.3892/ijmm.20.1.31
MLA
Konishi, M., Tanaka, H., Kaito, M., Fujita, N., Iwasa, M., Kobayashi, Y., Adachi, Y., Watanabe, S."Ultrastructural demonstration of hepatitis B virus production in a mouse model produced by hydrodynamic transfection". International Journal of Molecular Medicine 20.1 (2007): 31-36.
Chicago
Konishi, M., Tanaka, H., Kaito, M., Fujita, N., Iwasa, M., Kobayashi, Y., Adachi, Y., Watanabe, S."Ultrastructural demonstration of hepatitis B virus production in a mouse model produced by hydrodynamic transfection". International Journal of Molecular Medicine 20, no. 1 (2007): 31-36. https://doi.org/10.3892/ijmm.20.1.31
Copy and paste a formatted citation
x
Spandidos Publications style
Konishi M, Tanaka H, Kaito M, Fujita N, Iwasa M, Kobayashi Y, Adachi Y and Watanabe S: Ultrastructural demonstration of hepatitis B virus production in a mouse model produced by hydrodynamic transfection. Int J Mol Med 20: 31-36, 2007.
APA
Konishi, M., Tanaka, H., Kaito, M., Fujita, N., Iwasa, M., Kobayashi, Y. ... Watanabe, S. (2007). Ultrastructural demonstration of hepatitis B virus production in a mouse model produced by hydrodynamic transfection. International Journal of Molecular Medicine, 20, 31-36. https://doi.org/10.3892/ijmm.20.1.31
MLA
Konishi, M., Tanaka, H., Kaito, M., Fujita, N., Iwasa, M., Kobayashi, Y., Adachi, Y., Watanabe, S."Ultrastructural demonstration of hepatitis B virus production in a mouse model produced by hydrodynamic transfection". International Journal of Molecular Medicine 20.1 (2007): 31-36.
Chicago
Konishi, M., Tanaka, H., Kaito, M., Fujita, N., Iwasa, M., Kobayashi, Y., Adachi, Y., Watanabe, S."Ultrastructural demonstration of hepatitis B virus production in a mouse model produced by hydrodynamic transfection". International Journal of Molecular Medicine 20, no. 1 (2007): 31-36. https://doi.org/10.3892/ijmm.20.1.31
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