Novel embryonic stem cells expressing tdKaede protein photoconvertible from green to red fluorescence

  • Authors:
    • Yoko Shigematsu
    • Naoki Yoshida
    • Yoshihiro Miwa
    • Atsushi Mizobuti
    • Yuko Suzuki
    • Yoko Tanimoto
    • Satoru Takahashi
    • Satoshi Kunita
    • Fumihiro Sugiyama
    • Ken-ichi Yagami
  • View Affiliations

  • Published online on: October 1, 2007     https://doi.org/10.3892/ijmm.20.4.439
  • Pages: 439-444
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

Kaede protein is a photoconvertible tracer that emits green fluorescence after synthesis, which changes to stable red fluorescence upon irradiation with violet or UV illumination. This color-change characteristic is a very effective means of optically marking living cells of interest. We established novel embryonic stem (ES) cell lines, B6KED-1 and -2, from C57BL/6J transgenic mouse blastocysts ubiquitously expressing tandem dimeric Kaede (tdKaede) protein. Undifferentiated B6KED-1 and -2 cells showed bright green fluorescence and mRNAs of pluripotent marker genes. Photoconversion of tdKaede protein in undifferentiated and differentiated B6KED cells in vitro occurred upon short-term UV irradiation. B6KED cells completely generated ES cell-derived females on transfer into tetraploid blastomeres. All organs showed strong green emission in the females derived completely from B6KED cells. These novel ES cell lines ubiquitously expressing photoconvertible Kaede protein, B6KED-1 and -2, are useful for basic research in developmental biology and regenerative medicine.

Related Articles

Journal Cover

October 2007
Volume 20 Issue 4

Print ISSN: 1107-3756
Online ISSN:1791-244X

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
x
Spandidos Publications style
Shigematsu Y, Yoshida N, Miwa Y, Mizobuti A, Suzuki Y, Tanimoto Y, Takahashi S, Kunita S, Sugiyama F, Yagami K, Yagami K, et al: Novel embryonic stem cells expressing tdKaede protein photoconvertible from green to red fluorescence. Int J Mol Med 20: 439-444, 2007
APA
Shigematsu, Y., Yoshida, N., Miwa, Y., Mizobuti, A., Suzuki, Y., Tanimoto, Y. ... Yagami, K. (2007). Novel embryonic stem cells expressing tdKaede protein photoconvertible from green to red fluorescence. International Journal of Molecular Medicine, 20, 439-444. https://doi.org/10.3892/ijmm.20.4.439
MLA
Shigematsu, Y., Yoshida, N., Miwa, Y., Mizobuti, A., Suzuki, Y., Tanimoto, Y., Takahashi, S., Kunita, S., Sugiyama, F., Yagami, K."Novel embryonic stem cells expressing tdKaede protein photoconvertible from green to red fluorescence". International Journal of Molecular Medicine 20.4 (2007): 439-444.
Chicago
Shigematsu, Y., Yoshida, N., Miwa, Y., Mizobuti, A., Suzuki, Y., Tanimoto, Y., Takahashi, S., Kunita, S., Sugiyama, F., Yagami, K."Novel embryonic stem cells expressing tdKaede protein photoconvertible from green to red fluorescence". International Journal of Molecular Medicine 20, no. 4 (2007): 439-444. https://doi.org/10.3892/ijmm.20.4.439