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International Journal of Molecular Medicine
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Print ISSN: 1107-3756 Online ISSN: 1791-244X
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October 2007 Volume 20 Issue 4

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Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

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October 2007 Volume 20 Issue 4

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Article

Stable expression of the oxc and frc genes from Oxalobacter formigenes in human embryo kidney 293 cells: Implications for gene therapy of hyperoxaluria

  • Authors:
    • Zhang-Qun Ye
    • De-Bo Kong
    • Zhi-Qiang Chen
    • Lin-Fang Yao
    • Hui Guo
    • Xiao Yu
    • Guan-Lin Liu
    • Wei-Min Yang

  • View Affiliations / Copyright

    Affiliations: Department of Urology, Tongji Hospital, Tongji Medical School, Huazhong University of Science and Technology, Hubei 430030, P.R. China
  • Pages: 521-526
    |
    Published online on: October 1, 2007
       https://doi.org/10.3892/ijmm.20.4.521
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Abstract

Hyperoxaluria can lead to multiple pathologic conditions such as recurrent urolithiasis, oxalosis, nephrocalcinosis and even renal failure, but there is no known oxalate-degrading pathway in the human body, and current therapeutic options for patients with hyperoxaluria are limited. Oxalyl-CoA decarboxylase and formyl-CoA transferase are the key enzymes in the oxalate catabolism of Oxalobacter formigenes which dwell in the intestine of vertebrates and have an important symbiotic relationship with their hosts. The aim of this study was to insert the oxalate-degrading enzyme genes into human embryo kidney (HEK) 293 cells and to evaluate if the oxalate-degrading enzymes could be expressed in these cells and keep their enzyme activity. We present here the cloning of the two genes from O. formigenes and their subsequent expression in HEK293 cells. The results showed that the expression of oxalyl-CoA decarboxylase and formyl-CoA transferase was confirmed by RT-PCR and Western blotting, and the proteins were located in the cytoplasm of transfected cells. Furthermore, the transfected cells were capable to a certain degree of degrading oxalate. These findings suggest that the transfer of oxalate-degrading enzyme genes into human cells is possibly a potential candidate for the gene therapy of hyperoxaluria.

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Copy and paste a formatted citation
Spandidos Publications style
Ye Z, Kong D, Chen Z, Yao L, Guo H, Yu X, Liu G and Yang W: Stable expression of the oxc and frc genes from Oxalobacter formigenes in human embryo kidney 293 cells: Implications for gene therapy of hyperoxaluria. Int J Mol Med 20: 521-526, 2007.
APA
Ye, Z., Kong, D., Chen, Z., Yao, L., Guo, H., Yu, X. ... Yang, W. (2007). Stable expression of the oxc and frc genes from Oxalobacter formigenes in human embryo kidney 293 cells: Implications for gene therapy of hyperoxaluria. International Journal of Molecular Medicine, 20, 521-526. https://doi.org/10.3892/ijmm.20.4.521
MLA
Ye, Z., Kong, D., Chen, Z., Yao, L., Guo, H., Yu, X., Liu, G., Yang, W."Stable expression of the oxc and frc genes from Oxalobacter formigenes in human embryo kidney 293 cells: Implications for gene therapy of hyperoxaluria". International Journal of Molecular Medicine 20.4 (2007): 521-526.
Chicago
Ye, Z., Kong, D., Chen, Z., Yao, L., Guo, H., Yu, X., Liu, G., Yang, W."Stable expression of the oxc and frc genes from Oxalobacter formigenes in human embryo kidney 293 cells: Implications for gene therapy of hyperoxaluria". International Journal of Molecular Medicine 20, no. 4 (2007): 521-526. https://doi.org/10.3892/ijmm.20.4.521
Copy and paste a formatted citation
x
Spandidos Publications style
Ye Z, Kong D, Chen Z, Yao L, Guo H, Yu X, Liu G and Yang W: Stable expression of the oxc and frc genes from Oxalobacter formigenes in human embryo kidney 293 cells: Implications for gene therapy of hyperoxaluria. Int J Mol Med 20: 521-526, 2007.
APA
Ye, Z., Kong, D., Chen, Z., Yao, L., Guo, H., Yu, X. ... Yang, W. (2007). Stable expression of the oxc and frc genes from Oxalobacter formigenes in human embryo kidney 293 cells: Implications for gene therapy of hyperoxaluria. International Journal of Molecular Medicine, 20, 521-526. https://doi.org/10.3892/ijmm.20.4.521
MLA
Ye, Z., Kong, D., Chen, Z., Yao, L., Guo, H., Yu, X., Liu, G., Yang, W."Stable expression of the oxc and frc genes from Oxalobacter formigenes in human embryo kidney 293 cells: Implications for gene therapy of hyperoxaluria". International Journal of Molecular Medicine 20.4 (2007): 521-526.
Chicago
Ye, Z., Kong, D., Chen, Z., Yao, L., Guo, H., Yu, X., Liu, G., Yang, W."Stable expression of the oxc and frc genes from Oxalobacter formigenes in human embryo kidney 293 cells: Implications for gene therapy of hyperoxaluria". International Journal of Molecular Medicine 20, no. 4 (2007): 521-526. https://doi.org/10.3892/ijmm.20.4.521
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