Prion peptide-mediated cellular prion protein overexpression and neuronal cell death can be blocked by aspirin treatment

  • Authors:
    • Jae-Kyo Jeong
    • Myung-Hee Moon
    • Jae-Won Seol
    • Jae-Suk Seo
    • You-Jin Lee
    • Sang-Youel Park
  • View Affiliations

  • Published online on: February 23, 2011     https://doi.org/10.3892/ijmm.2011.626
  • Pages: 689-693
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Abstract

Prion diseases are infectious neurodegenerative disorders characterized by the conversion of the cellular prion protein (PrPc) to the misfolded isoform (PrPsc). Prion peptide PrP 106‑126 [PrP (106‑126)] shares many physiological properties with PrPsc; it is neurotoxic in vitro and in vivo. PrP (106‑126) induces neurotoxicity by the overexpression of PrPc and activation of the mitogen-activated protein (ERK1/2). Aspirin, an anti-inflammatory drug, is a known ERK inhibitor and prevents neurodegenerative disorders including prion diseases. The influence of aspirin treatment on prion protein-mediated neurotoxicity and expression of PrPc were the focus of this study. Cell viability and apoptosis were assessed by crystal violet staining and the TUNEL and DNA fragmentation assays. Apoptosis-associated protein expression of PrPc, p-53, p-ERK1/2, p-p38, Bcl-2 and cleaved-caspase-3 was examined by Western blotting and immunocytochemistry. Aspirin treatment inhibited PrP (106‑126)-induced neuronal cell death in SH-SY5Y neuroblastoma cells. In addition, the PrP (106‑126)-mediated increase of p-p38, p53, cleaved-caspase-3 and decrease of Bcl-2 expressions were blocked by aspirin and the ERK inhibitor, PR98059. Furthermore, we showed that the PrP (106‑126)-mediated increase of PrPc and p-ERK1/2 were inhibited by PD98059 and aspirin. Taken together, these results demonstrate that ERK1/2 is a key modulator of the protective effect of aspirin on PrP-106‑126-mediated cellular prion protein overexpression and neurotoxicity and also suggest that aspirin may prevent neuron cell damages caused by the prion peptide.

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May 2011
Volume 27 Issue 5

Print ISSN: 1107-3756
Online ISSN:1791-244X

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Spandidos Publications style
Jeong J, Moon M, Seol J, Seo J, Lee Y and Park S: Prion peptide-mediated cellular prion protein overexpression and neuronal cell death can be blocked by aspirin treatment. Int J Mol Med 27: 689-693, 2011
APA
Jeong, J., Moon, M., Seol, J., Seo, J., Lee, Y., & Park, S. (2011). Prion peptide-mediated cellular prion protein overexpression and neuronal cell death can be blocked by aspirin treatment. International Journal of Molecular Medicine, 27, 689-693. https://doi.org/10.3892/ijmm.2011.626
MLA
Jeong, J., Moon, M., Seol, J., Seo, J., Lee, Y., Park, S."Prion peptide-mediated cellular prion protein overexpression and neuronal cell death can be blocked by aspirin treatment". International Journal of Molecular Medicine 27.5 (2011): 689-693.
Chicago
Jeong, J., Moon, M., Seol, J., Seo, J., Lee, Y., Park, S."Prion peptide-mediated cellular prion protein overexpression and neuronal cell death can be blocked by aspirin treatment". International Journal of Molecular Medicine 27, no. 5 (2011): 689-693. https://doi.org/10.3892/ijmm.2011.626