Intracoronary adiponectin at reperfusion reduces infarct size in a porcine myocardial infarction model

  • Authors:
    • Marcin Dębiński
    • Piotr P. Buszman
    • Krzysztof Milewski
    • Wojciech Wojakowski
    • Wanda Jackiewicz
    • Jacek Pająk
    • Dariusz Szurlej
    • Joanna Fryc-Stanek
    • Szymon Wiernek
    • Michal Jelonek
    • Michael E. Spurlock
    • Jack Martin
    • Andrzej Bochenek
    • Pawel E. Buszman
  • View Affiliations

  • Published online on: March 8, 2011     https://doi.org/10.3892/ijmm.2011.643
  • Pages: 775-781
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Abstract

Reperfusion injury (RI) remains an important limitation of myocardial revascularization. The aim of the present study was to evaluate the influence of the intracoronary injection of adiponectin on RI and cardiomyocyte death in a porcine myocardial infarction model. Acute infarction in 14 Polish domestic pigs was induced by inflation of an over the wire balloon (OTW) catheter in the medial left anterior descending artery for 60 min. The study group consisted of 7 pigs in which intracoronary adiponectin (50 µg) was infused through the OTW catheter immediately before reperfusion. The control group (n=7) was administered placebo. Animals were sacrificed after two days of follow-up. The infarct area (IA) was stained with tetrazoline and the area at risk (AAR) with intracoronary administration of Evans Blue dye before euthanasia. Hearts in each group had similar AARs (46.2±9.9% vs. 48.4±6.2% of the whole myocardium, p=ns). The IA/AAR% and IA were smaller in the study group when compared to the control (24.7±4.0% vs. 45.3±22.5%, p=0.005; and 11.7±4.9% vs. 20.5±5.6%, p=0.01, respectively). These outcomes corresponded well with the peak troponin levels after 12 h (109.9±60.9 ng/ml vs. 185.5±39.4 ng/ml, p=0.017). After two days there was a significantly higher LVEF in the study group (51.4±8.5% vs. 33.9±8.6%, p=0.002). There was also a trend toward lower apoptosis enhancement in the viable myocardium in the study group (3.11±2.3 vs. 8.92±6.3; p=0.07). The administration of adiponectin into the infarct- related artery is safe and feasible. The treatment significantly reduced the infarct size.

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June 2011
Volume 27 Issue 6

Print ISSN: 1107-3756
Online ISSN:1791-244X

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Spandidos Publications style
Dębiński M, Buszman PP, Milewski K, Wojakowski W, Jackiewicz W, Pająk J, Szurlej D, Fryc-Stanek J, Wiernek S, Jelonek M, Jelonek M, et al: Intracoronary adiponectin at reperfusion reduces infarct size in a porcine myocardial infarction model. Int J Mol Med 27: 775-781, 2011
APA
Dębiński, M., Buszman, P.P., Milewski, K., Wojakowski, W., Jackiewicz, W., Pająk, J. ... Buszman, P.E. (2011). Intracoronary adiponectin at reperfusion reduces infarct size in a porcine myocardial infarction model. International Journal of Molecular Medicine, 27, 775-781. https://doi.org/10.3892/ijmm.2011.643
MLA
Dębiński, M., Buszman, P. P., Milewski, K., Wojakowski, W., Jackiewicz, W., Pająk, J., Szurlej, D., Fryc-Stanek, J., Wiernek, S., Jelonek, M., Spurlock, M. E., Martin, J., Bochenek, A., Buszman, P. E."Intracoronary adiponectin at reperfusion reduces infarct size in a porcine myocardial infarction model". International Journal of Molecular Medicine 27.6 (2011): 775-781.
Chicago
Dębiński, M., Buszman, P. P., Milewski, K., Wojakowski, W., Jackiewicz, W., Pająk, J., Szurlej, D., Fryc-Stanek, J., Wiernek, S., Jelonek, M., Spurlock, M. E., Martin, J., Bochenek, A., Buszman, P. E."Intracoronary adiponectin at reperfusion reduces infarct size in a porcine myocardial infarction model". International Journal of Molecular Medicine 27, no. 6 (2011): 775-781. https://doi.org/10.3892/ijmm.2011.643