Endothelin-1 does not impair insulin-induced angiogenesis in vitro

  • Authors:
    • Fabio Pellegatta
    • Claudia Brambilla
    • Alice Reduzzi
    • Marta Bragheri
    • Gianpaolo Zerbini
    • Alberico L. Catapano
  • View Affiliations

  • Published online on: May 2, 2011     https://doi.org/10.3892/ijmm.2011.689
  • Pages: 443-448
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

Endothelin-1 (ET-1) modulates several vascular functions and plays an important role in the pathogenesis of insulin resistance. However, its role in the pathogenesis of impaired angiogenesis observed under insulin resistance conditions is not known. In the present study, we addressed this issue by analyzing the effect of ET-1 in human umbilical vein endothelial cells (HUVEC) on i) insulin-induced phosphorylation of two protein kinases involved in angiogenesis, Akt and ERK1/2, and on ii) insulin-induced angiogenesis in two in vitro models, those of Matrigel and of fibroblast/endothelial co-culture. Both insulin (100 ng/ml) and ET-1 (10 nmol/l) dose-dependently increased the phosphorylation of Akt and ERK1/2. Pre-treatment with ET-1 did not suppress the insulin-induced Akt and ERK1/2 phosphorylation. In the two in vitro models of angiogenesis, ET-1 did not inhibit insulin-induced angiogenesis. From these data we conclude that in vitro, at the times and at the concentrations examined, ET-1 does not impair insulin-induced angiogenesis.

Related Articles

Journal Cover

September 2011
Volume 28 Issue 3

Print ISSN: 1107-3756
Online ISSN:1791-244X

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
x
Spandidos Publications style
Pellegatta F, Brambilla C, Reduzzi A, Bragheri M, Zerbini G and Catapano AL: Endothelin-1 does not impair insulin-induced angiogenesis in vitro . Int J Mol Med 28: 443-448, 2011
APA
Pellegatta, F., Brambilla, C., Reduzzi, A., Bragheri, M., Zerbini, G., & Catapano, A.L. (2011). Endothelin-1 does not impair insulin-induced angiogenesis in vitro . International Journal of Molecular Medicine, 28, 443-448. https://doi.org/10.3892/ijmm.2011.689
MLA
Pellegatta, F., Brambilla, C., Reduzzi, A., Bragheri, M., Zerbini, G., Catapano, A. L."Endothelin-1 does not impair insulin-induced angiogenesis in vitro ". International Journal of Molecular Medicine 28.3 (2011): 443-448.
Chicago
Pellegatta, F., Brambilla, C., Reduzzi, A., Bragheri, M., Zerbini, G., Catapano, A. L."Endothelin-1 does not impair insulin-induced angiogenesis in vitro ". International Journal of Molecular Medicine 28, no. 3 (2011): 443-448. https://doi.org/10.3892/ijmm.2011.689