Open Access

A glycoprotein from Porphyra yezoensis produces anti-inflammatory effects in liposaccharide-stimulated macrophages via the TLR4 signaling pathway

  • Authors:
    • Eun-Soon Shin
    • Hye-Jung Hwang
    • In-Hye Kim
    • Taek-Jeong Nam
  • View Affiliations

  • Published online on: June 23, 2011     https://doi.org/10.3892/ijmm.2011.729
  • Pages: 809-815
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Abstract

The purpose of this study was to investigate the antioxidant and anti-inflammatory effects of a glycoprotein isolated from the alga Porphyra yezoensis in LPS-stimulated RAW 264.7 mouse macrophages. First, we extracted a novel material with antioxidant activity from P. yezoensis, confirmed by SDS-PAGE to be a glycoprotein, which we named P. yezoensis glycoprotein (PGP). PGP inhibited the production of NO and ROS and expression of iNOS, COX-2, TNF-α and IL-1β, which are involved in the pathogenesis of many inflammation-associated human diseases, including septic shock, hemorrhagic shock and rheumatoid arthritis. Next, we determined the mechanisms behind the antioxidant and anti-inflammatory activities of PGP. We focused on the Toll-like receptor 4 (TLR4) signaling pathway because it is well-known to induce the pro-inflammatory proteins that trigger MAPK and NF-κB activation in lipopolysaccharide (LPS)-induced oxidative events. PGP treatment reduced the formation of the TLR4-IRAK4 and TLR4-TRIF binding complexes in response to LPS. Moreover, it inhibited LPS-induced activation and nuclear translocation of NF-κB by abrogating IκB phosphorylation. PGP also suppressed the phosphorylation of ERK1/2 and JNK in a dose-dependent manner. These results suggest that PGP exerts its anti-inflammatory effects by modulating TLR4 signaling and thus inhibiting the activation of NF-κB and MAP kinases.

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November 2011
Volume 28 Issue 5

Print ISSN: 1107-3756
Online ISSN:1791-244X

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Spandidos Publications style
Shin E, Hwang H, Kim I and Nam T: A glycoprotein from Porphyra yezoensis produces anti-inflammatory effects in liposaccharide-stimulated macrophages via the TLR4 signaling pathway. Int J Mol Med 28: 809-815, 2011
APA
Shin, E., Hwang, H., Kim, I., & Nam, T. (2011). A glycoprotein from Porphyra yezoensis produces anti-inflammatory effects in liposaccharide-stimulated macrophages via the TLR4 signaling pathway. International Journal of Molecular Medicine, 28, 809-815. https://doi.org/10.3892/ijmm.2011.729
MLA
Shin, E., Hwang, H., Kim, I., Nam, T."A glycoprotein from Porphyra yezoensis produces anti-inflammatory effects in liposaccharide-stimulated macrophages via the TLR4 signaling pathway". International Journal of Molecular Medicine 28.5 (2011): 809-815.
Chicago
Shin, E., Hwang, H., Kim, I., Nam, T."A glycoprotein from Porphyra yezoensis produces anti-inflammatory effects in liposaccharide-stimulated macrophages via the TLR4 signaling pathway". International Journal of Molecular Medicine 28, no. 5 (2011): 809-815. https://doi.org/10.3892/ijmm.2011.729