Open Access

An anti-HIV-1 compound that increases steady-state expression of apoplipoprotein B mRNA-editing enzyme-catalytic polypeptide-like 3G

  • Authors:
    • Tomohiko Ejima
    • Mayuko Hirota
    • Tamio Mizukami
    • Masami Otsuka
    • Mikako Fujita
  • View Affiliations

  • Published online on: Friday, July 1, 2011
  • Pages: 613-616 DOI: 10.3892/ijmm.2011.737
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Abstract

Human apoplipoprotein B mRNA-editing enzyme-catalytic polypeptide-like (APOBEC) 3G (A3G) is an antiviral protein that blocks HIV-1 replication. However, the antiviral activity of A3G is overcome by the HIV-1 protein Vif. This inhibitory function of Vif is related to its ability to degrade A3G in the proteasome. This finding prompted us to examine the activities of 4-(dimethylamino)-2,6-bis[(N-(2-[(2-nitrophenyl)dithio]ethyl)amino)methyl]pyridine (SN-2) and SN-3. We found that 5 µM SN-2 increases the expression of A3G to a level much higher than that observed in the absence of Vif, without affecting the level of Vif expression. The proteasome inhibitor MG-132 increased the level of both A3G and Vif expression. These results demonstrate that A3G is ubiquitinated and degraded in the proteasome by a factor other than Vif, and that SN-2 selectively inhibits these processes. Furthermore, 5 µM SN-2 significantly inhibited the MAGI cell infectivity of wild-type HIV-1. These findings may contribute to the development of a novel anti-HIV-1 drug.

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Journal Cover

October 2011
Volume 28 Issue 4

Print ISSN: 1107-3756
Online ISSN:1791-244X

2015 Impact Factor: 2.348
Ranked #22/123 Medicine Research and Experimental
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APA
Ejima, T., Hirota, M., Mizukami, T., Otsuka, M., & Fujita, M. (2011). An anti-HIV-1 compound that increases steady-state expression of apoplipoprotein B mRNA-editing enzyme-catalytic polypeptide-like 3G. International Journal of Molecular Medicine, 28, 613-616. http://dx.doi.org/10.3892/ijmm.2011.737
MLA
Ejima, T., Hirota, M., Mizukami, T., Otsuka, M., Fujita, M."An anti-HIV-1 compound that increases steady-state expression of apoplipoprotein B mRNA-editing enzyme-catalytic polypeptide-like 3G". International Journal of Molecular Medicine 28.4 (2011): 613-616.
Chicago
Ejima, T., Hirota, M., Mizukami, T., Otsuka, M., Fujita, M."An anti-HIV-1 compound that increases steady-state expression of apoplipoprotein B mRNA-editing enzyme-catalytic polypeptide-like 3G". International Journal of Molecular Medicine 28, no. 4 (2011): 613-616. http://dx.doi.org/10.3892/ijmm.2011.737