Oxygen- and glucose-deprived culture promotes cell proliferation and invasion of vascular smooth muscle cells

  • Authors:
    • Liang Meng
    • Bo Yu
  • View Affiliations

  • Published online on: July 8, 2011     https://doi.org/10.3892/ijmm.2011.744
  • Pages: 777-783
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

Excessive proliferation of vascular smooth muscle cells (VSMCs) is an important reason for the formation and development of many vascular remodeling diseases. In pathological conditions, necrosis of VSMCs may result in the release of inflammatory cytokines, which can lead to stimulation of other normal smooth muscle cells, and promote the proliferation of VSMCs. The purpose of this study was to investigate the effect of oxygen- and glucose-deprived (OGD) conditioned medium on VSMC cell proliferation and invasion. Following culture of VSMCs in OGD-conditioned medium, the cell cycle distributions were remarkably altered. The number of cells in the G0/G1 phase decreased, while the number of cells in G2/M and S phase increased. The expression of cell cycle proteins D1 (Cyclin D1) in VSMCs increased correspondingly. These results suggested that after being cultured in OGD medium, VSMCs can pass through the G0/G1 phase by up-regulation of Cyclin D1 expression, and promote cell proliferation. In addition, we found that the expression of matrix metalloproteinase (MMP)-2 and MMP-9 was increased in OGD medium cultured VSMCs. Using a Transwell invasion assay, we showed that the OGD medium enhanced VSMC cell invasion. These results suggest that MMP-2 and MMP-9 degraded the basement membrane and promoted VSMC invasion. Taken together, our data demonstrate that OGD-conditioned medium can promote VSMC proliferation and invasion by up-regulating Cyclin D1 and MMP-2 and MMP-9 expression, which may contribute to the formation and development of vascular remodeling diseases.

Related Articles

Journal Cover

November 2011
Volume 28 Issue 5

Print ISSN: 1107-3756
Online ISSN:1791-244X

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
x
Spandidos Publications style
Meng L and Meng L: Oxygen- and glucose-deprived culture promotes cell proliferation and invasion of vascular smooth muscle cells. Int J Mol Med 28: 777-783, 2011
APA
Meng, L., & Meng, L. (2011). Oxygen- and glucose-deprived culture promotes cell proliferation and invasion of vascular smooth muscle cells. International Journal of Molecular Medicine, 28, 777-783. https://doi.org/10.3892/ijmm.2011.744
MLA
Meng, L., Yu, B."Oxygen- and glucose-deprived culture promotes cell proliferation and invasion of vascular smooth muscle cells". International Journal of Molecular Medicine 28.5 (2011): 777-783.
Chicago
Meng, L., Yu, B."Oxygen- and glucose-deprived culture promotes cell proliferation and invasion of vascular smooth muscle cells". International Journal of Molecular Medicine 28, no. 5 (2011): 777-783. https://doi.org/10.3892/ijmm.2011.744