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International Journal of Molecular Medicine
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Print ISSN: 1107-3756 Online ISSN: 1791-244X
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October 2011 Volume 28 Issue 4

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International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

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Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

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International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

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International Journal of Epigenetics

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Article

Rho-kinase regulates thrombin-stimulated interleukin-6 synthesis via p38 mitogen-activated protein kinase in osteoblasts

  • Authors:
    • Kenji Kato
    • Takanobu Otsuka
    • Rie Matsushima-Nishiwaki
    • Hideo Natsume
    • Osamu Kozawa
    • Haruhiko Tokuda

  • View Affiliations / Copyright

    Affiliations: Department of Orthopedic Surgery, Nagoya City University Graduate School of Medical Sciences, Nagoya 467-8601, Japan, Department of Clinical Laboratory, National Center for Geriatrics and Gerontology, Obu 474-8511, Japan
  • Pages: 653-658
    |
    Published online on: July 12, 2011
       https://doi.org/10.3892/ijmm.2011.747
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Abstract

We have previously reported that thrombin stimulates synthesis of interleukin-6 (IL-6), a potent bone resorptive agent, in osteoblast-like MC3T3-E1 cells. In the present study, we investigated the mechanism of thrombin in the thrombin-stimulated IL-6 synthesis and the involvement of Rho-kinase in MC3T3-E1 cells. Thrombin time-dependently induced the phosphorylation of p44/p42 mitogen-activated protein (MAP) kinase, p38 MAP kinase, stress-activated protein kinase/c-Jun N-terminal kinase (SAPK/JNK) and myosin phosphatase targeting subunit-1 (MYPT-1), a Rho-kinase substrate. While SP600125, an inhibitor of SAPK/JNK, failed to reduce IL-6 synthesis, PD98059, a specific inhibitor of MEK, and SB203580 and BIRB0796, potent inhibitors of p38 MAP kinase, suppressed the IL-6 synthesis induced by thrombin. Y27632, a specific Rho-kinase inhibitor, significantly reduced thrombin-stimulated IL-6 synthesis as well as the MYPT-1 phosphorylation. Fasudil, another inhibitor of Rho-kinase, suppressed thrombin-stimulated IL-6 synthesis. Y27632 and fasudil failed to affect thrombin-induced phosphorylation of p44/p42 MAP kinase. Y27632 as well as fasudil attenuated thrombin-induced phosphorylation of p38 MAP kinase. These results strongly suggest that Rho-kinase regulates thrombin-stimulated IL-6 synthesis via p38 MAP kinase activation in osteoblasts.

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Copy and paste a formatted citation
Spandidos Publications style
Kato K, Otsuka T, Matsushima-Nishiwaki R, Natsume H, Kozawa O and Tokuda H: Rho-kinase regulates thrombin-stimulated interleukin-6 synthesis via p38 mitogen-activated protein kinase in osteoblasts. Int J Mol Med 28: 653-658, 2011.
APA
Kato, K., Otsuka, T., Matsushima-Nishiwaki, R., Natsume, H., Kozawa, O., & Tokuda, H. (2011). Rho-kinase regulates thrombin-stimulated interleukin-6 synthesis via p38 mitogen-activated protein kinase in osteoblasts. International Journal of Molecular Medicine, 28, 653-658. https://doi.org/10.3892/ijmm.2011.747
MLA
Kato, K., Otsuka, T., Matsushima-Nishiwaki, R., Natsume, H., Kozawa, O., Tokuda, H."Rho-kinase regulates thrombin-stimulated interleukin-6 synthesis via p38 mitogen-activated protein kinase in osteoblasts". International Journal of Molecular Medicine 28.4 (2011): 653-658.
Chicago
Kato, K., Otsuka, T., Matsushima-Nishiwaki, R., Natsume, H., Kozawa, O., Tokuda, H."Rho-kinase regulates thrombin-stimulated interleukin-6 synthesis via p38 mitogen-activated protein kinase in osteoblasts". International Journal of Molecular Medicine 28, no. 4 (2011): 653-658. https://doi.org/10.3892/ijmm.2011.747
Copy and paste a formatted citation
x
Spandidos Publications style
Kato K, Otsuka T, Matsushima-Nishiwaki R, Natsume H, Kozawa O and Tokuda H: Rho-kinase regulates thrombin-stimulated interleukin-6 synthesis via p38 mitogen-activated protein kinase in osteoblasts. Int J Mol Med 28: 653-658, 2011.
APA
Kato, K., Otsuka, T., Matsushima-Nishiwaki, R., Natsume, H., Kozawa, O., & Tokuda, H. (2011). Rho-kinase regulates thrombin-stimulated interleukin-6 synthesis via p38 mitogen-activated protein kinase in osteoblasts. International Journal of Molecular Medicine, 28, 653-658. https://doi.org/10.3892/ijmm.2011.747
MLA
Kato, K., Otsuka, T., Matsushima-Nishiwaki, R., Natsume, H., Kozawa, O., Tokuda, H."Rho-kinase regulates thrombin-stimulated interleukin-6 synthesis via p38 mitogen-activated protein kinase in osteoblasts". International Journal of Molecular Medicine 28.4 (2011): 653-658.
Chicago
Kato, K., Otsuka, T., Matsushima-Nishiwaki, R., Natsume, H., Kozawa, O., Tokuda, H."Rho-kinase regulates thrombin-stimulated interleukin-6 synthesis via p38 mitogen-activated protein kinase in osteoblasts". International Journal of Molecular Medicine 28, no. 4 (2011): 653-658. https://doi.org/10.3892/ijmm.2011.747
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