Azacitidine induces demethylation of p16INK4a and inhibits growth in adult T-cell leukemia/lymphoma

  • Authors:
    • Kumi Uenogawa
    • Yoshihiro Hatta
    • Naomichi Arima
    • Satoshi Hayakawa
    • Umihiko Sawada
    • Shin Aizawa
    • Tatsuo Yamamoto
    • Jin Takeuchi
  • View Affiliations

  • Published online on: July 22, 2011     https://doi.org/10.3892/ijmm.2011.756
  • Pages: 835-839
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Abstract

Adult T-cell leukemia/lymphoma (ATL) is one of the peripheral T-cell malignant neoplasms strongly associated with human T-cell leukemia virus type-I (HTLV-I). Although the viral transactivator protein Tax has been proposed to play a critical role in leukemogeneis, additional cellular events are required for the development of ATL. One of the genetic events of the disease is inactivation of tumor suppressor genes. The CDKN2A locus on chromosome 9p encodes 2 cell cycle regulatory proteins, p14ARF and p16INK4a, which share exon 2 using different reading frames. The p14ARF and p16INK4a genes have been implicated as tumor suppressor genes by their frequent mutation, deletion or promoter hypermethylation in a variety of human tumors. In this report, we describe the expression status of p14ARF and p16INK4a in 9 ATL cell lines (MT1, MT2, OKM3T, F6T, K3T, Oh13T, S1T, Su9T01 and HUT102). By reverse transcription polymerase chain reaction (RT-PCR), expression of p14ARF was not detected in one cell line (OKM3T), while expression of p16INK4a was not detected in 6 cell lines (OKM3T, MT1, MT2, Oh13T, S1T and Su9T01). In the OKM3T cell line, the shared exon 2 of the p14ARF/p16INK4a gene was deleted; however, the p16INK4a gene, was epigenetically inactivated in 5 other cells lines. In primary tumor cells obtained from ATL patients, p14ARF expression was absent in 6 of the 11 samples. We confirmed the methylation of the p16INK4a gene in MT1 and MT2 cells using the methylation-specific PCR (MSP) method. Treatment with 2.0 µM of Azacitidine (AZA), a demethylating agent, for 72 h restored p16INK4a transcript expression and induced growth inhibition in MT2 cells. Our results demonstrate that p16INK4a is epigenetically silenced in ATL. AZA offers a potential new therapeutic approach to improve the poor outcomes associated with ATL.

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November 2011
Volume 28 Issue 5

Print ISSN: 1107-3756
Online ISSN:1791-244X

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Spandidos Publications style
Uenogawa K, Hatta Y, Arima N, Hayakawa S, Sawada U, Aizawa S, Yamamoto T and Takeuchi J: Azacitidine induces demethylation of p16INK4a and inhibits growth in adult T-cell leukemia/lymphoma. Int J Mol Med 28: 835-839, 2011
APA
Uenogawa, K., Hatta, Y., Arima, N., Hayakawa, S., Sawada, U., Aizawa, S. ... Takeuchi, J. (2011). Azacitidine induces demethylation of p16INK4a and inhibits growth in adult T-cell leukemia/lymphoma. International Journal of Molecular Medicine, 28, 835-839. https://doi.org/10.3892/ijmm.2011.756
MLA
Uenogawa, K., Hatta, Y., Arima, N., Hayakawa, S., Sawada, U., Aizawa, S., Yamamoto, T., Takeuchi, J."Azacitidine induces demethylation of p16INK4a and inhibits growth in adult T-cell leukemia/lymphoma". International Journal of Molecular Medicine 28.5 (2011): 835-839.
Chicago
Uenogawa, K., Hatta, Y., Arima, N., Hayakawa, S., Sawada, U., Aizawa, S., Yamamoto, T., Takeuchi, J."Azacitidine induces demethylation of p16INK4a and inhibits growth in adult T-cell leukemia/lymphoma". International Journal of Molecular Medicine 28, no. 5 (2011): 835-839. https://doi.org/10.3892/ijmm.2011.756