Epigenetic signatures of somatic cell nuclear transfer-derived embryonic stem cells

  • Authors:
    • Eui-Man Jung
    • Hye Young Son
    • Min Kwon Jeung
    • Chang Kyu Lee
    • Sang Hwan Hyun
    • Eui-Bae Jeung
  • View Affiliations

  • Published online on: August 11, 2011     https://doi.org/10.3892/ijmm.2011.772
  • Pages: 697-704
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Abstract

Some human embryonic stem cell lines have shown genomic instabilities over long-term culture. To study the controversial origin of the SCNT-hES-1 line, which was derived from autologous somatic cell nuclear transfer (SCNT), we compared the expression and methylation patterns of imprinted genes in the SCNT-hES-1 cells with the donor's somatic cells by semi-quantitative RT-PCR, real-time PCR and bisulfite sequencing. Examined imprinted genes were H19, GNAS, SLC22A18, UBE3A and ZNF264 for maternally expressed genes, and IGF2, SNRPN, PEG3, PEG10, MEST, MAGEL2 and ARHI for paternally expressed genes, respectively. We found that the expression of imprinted genes in the SCNT-hES-1 cell line is comparable to that in the donor's somatic cells, and that its methylation patterns are similar to those of other SCNT-products. Therefore, the present study indicates that the SCNT-hES-1 line was derived from SCNT.

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November 2011
Volume 28 Issue 5

Print ISSN: 1107-3756
Online ISSN:1791-244X

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Spandidos Publications style
Jung E, Son HY, Jeung MK, Lee CK, Hyun SH and Jeung E: Epigenetic signatures of somatic cell nuclear transfer-derived embryonic stem cells. Int J Mol Med 28: 697-704, 2011
APA
Jung, E., Son, H.Y., Jeung, M.K., Lee, C.K., Hyun, S.H., & Jeung, E. (2011). Epigenetic signatures of somatic cell nuclear transfer-derived embryonic stem cells. International Journal of Molecular Medicine, 28, 697-704. https://doi.org/10.3892/ijmm.2011.772
MLA
Jung, E., Son, H. Y., Jeung, M. K., Lee, C. K., Hyun, S. H., Jeung, E."Epigenetic signatures of somatic cell nuclear transfer-derived embryonic stem cells". International Journal of Molecular Medicine 28.5 (2011): 697-704.
Chicago
Jung, E., Son, H. Y., Jeung, M. K., Lee, C. K., Hyun, S. H., Jeung, E."Epigenetic signatures of somatic cell nuclear transfer-derived embryonic stem cells". International Journal of Molecular Medicine 28, no. 5 (2011): 697-704. https://doi.org/10.3892/ijmm.2011.772