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International Journal of Molecular Medicine
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Print ISSN: 1107-3756 Online ISSN: 1791-244X
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January 2012 Volume 29 Issue 1

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Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

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Article

Evaluation of CMV and KAP promoters for driving the expression of human CYP4F2 in transgenic mice

  • Authors:
    • Guangrui Lai
    • Xiaoliang Liu
    • Jingjing Wu
    • Hong Liu
    • Yanyan Zhao

  • View Affiliations / Copyright

    Affiliations: Department of Clinical Genetics, Shengjing Hospital of China Medical University, Shenyang, Liaoning, P.R. China, Department of Clinical Genetics, Shengjing Hospital of China Medical University, 36 San Hao Street, Shenyang, Liaoning 110004, P.R. China
  • Pages: 107-112
    |
    Published online on: September 1, 2011
       https://doi.org/10.3892/ijmm.2011.787
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Abstract

A transgenic mouse model in which cytochrome P450 4F2 (CYP4F2) was expressed in multiple organs was expected to clarify the role of 20-hydroxyeicosatetraenoic acid (20-HETE) in the regulation of blood pressure, compared with our previously established kidney androgen-regulated protein (KAP) promoter CYP4F2 transgenic mouse model which predominantly showed renal overexpression of CYP4F2. A novel CYP4F2 transgenic mouse model driven by the cyto­megalovirus (CMV) promoter was generated and identified by PCR and subsequent sequencing. Extensive study of CMV-CYP4F2 transgenic mice demonstrated that CYP4F2 was exclusively expressed in the liver, while 20-HETE levels in the urine, kidney and blood were not affected, and there was no resulting change in the systolic blood pressure. This was in contrast to KAP-CYP4F2 transgenic mice which exerted prohypertensive action of 20-HETE resulting from the renal overexpression of CYP4F2. In addition, CYP4F2 overwhelmed the endogenous renal Cyp4a family mRNA levels in the KAP-CYP4F2 but not in the CMV-CYP4F2 transgenic mice. These results support the idea that overexpression of renal CYP4F2, leading to high 20-HETE in the urine and blood, may account for the elevated blood pressure. The CMV promoter did not direct CYP4F2 expression into extensive tissues and organs in an attempt to clarify the action of 20-HETE.

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Copy and paste a formatted citation
Spandidos Publications style
Lai G, Liu X, Wu J, Liu H and Zhao Y: Evaluation of CMV and KAP promoters for driving the expression of human CYP4F2 in transgenic mice. Int J Mol Med 29: 107-112, 2012.
APA
Lai, G., Liu, X., Wu, J., Liu, H., & Zhao, Y. (2012). Evaluation of CMV and KAP promoters for driving the expression of human CYP4F2 in transgenic mice. International Journal of Molecular Medicine, 29, 107-112. https://doi.org/10.3892/ijmm.2011.787
MLA
Lai, G., Liu, X., Wu, J., Liu, H., Zhao, Y."Evaluation of CMV and KAP promoters for driving the expression of human CYP4F2 in transgenic mice". International Journal of Molecular Medicine 29.1 (2012): 107-112.
Chicago
Lai, G., Liu, X., Wu, J., Liu, H., Zhao, Y."Evaluation of CMV and KAP promoters for driving the expression of human CYP4F2 in transgenic mice". International Journal of Molecular Medicine 29, no. 1 (2012): 107-112. https://doi.org/10.3892/ijmm.2011.787
Copy and paste a formatted citation
x
Spandidos Publications style
Lai G, Liu X, Wu J, Liu H and Zhao Y: Evaluation of CMV and KAP promoters for driving the expression of human CYP4F2 in transgenic mice. Int J Mol Med 29: 107-112, 2012.
APA
Lai, G., Liu, X., Wu, J., Liu, H., & Zhao, Y. (2012). Evaluation of CMV and KAP promoters for driving the expression of human CYP4F2 in transgenic mice. International Journal of Molecular Medicine, 29, 107-112. https://doi.org/10.3892/ijmm.2011.787
MLA
Lai, G., Liu, X., Wu, J., Liu, H., Zhao, Y."Evaluation of CMV and KAP promoters for driving the expression of human CYP4F2 in transgenic mice". International Journal of Molecular Medicine 29.1 (2012): 107-112.
Chicago
Lai, G., Liu, X., Wu, J., Liu, H., Zhao, Y."Evaluation of CMV and KAP promoters for driving the expression of human CYP4F2 in transgenic mice". International Journal of Molecular Medicine 29, no. 1 (2012): 107-112. https://doi.org/10.3892/ijmm.2011.787
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