Elevated expression of transforming growth factor β3 in carbon tetrachloride-treated rat liver and involvement of retinoid signaling

Mezaki,Yoshihiro; Morii,Mayako; Yoshikawa,Kiwamu; Yamaguchi,Noriko; Satoyoshi,Kiyofumi; Miura,Mitsutaka; Imai,Katsuyuki; Hebiguchi,Tatsuzo; Habuchi,Tomonori; Senoo,Haruki

doi:10.3892/ijmm.2011.809

January 2012 Volume 29 Issue 1

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January 2012 Volume 29 Issue 1

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Article

Elevated expression of transforming growth factor β3 in carbon tetrachloride-treated rat liver and involvement of retinoid signaling

Authors:
- Yoshihiro Mezaki
- Mayako Morii
- Kiwamu Yoshikawa
- Noriko Yamaguchi
- Kiyofumi Satoyoshi
- Mitsutaka Miura
- Katsuyuki Imai
- Tatsuzo Hebiguchi
- Tomonori Habuchi
- Haruki Senoo
View Affiliations / Copyright

Affiliations: Department of Cell Biology and Morphology, Akita University Graduate School of Medicine, 1-1-1 Hondo, Akita 010-8543, Japan
Pages: 18-24
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Published online on: October 6, 2011

https://doi.org/10.3892/ijmm.2011.809
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Abstract

Transforming growth factor (TGF) β is a pro-fibrotic cytokine. While three isoforms (TGF-β1, 2 and 3) are known, the functional differences between them are obscure. To investigate the roles of TGF-β isoforms during liver fibrogenesis, male Wistar rats were administrated carbon tetrachloride (CCl4) subcutaneously twice a week for two months. Livers were excised and sectioned for histochemical examinations. These livers were also used to quantitate the expression of genes associated with fibrogenesis, including TGF-β isoforms, as well as those associated with retinoid metabolism. Expression levels of Tgfb1 and Tgfb3 were up-regulated in CCl4-treated rat livers while that of Tgfb2 was not changed. The mRNAs for lecithin-retinol acyltransferase (Lrat) and retinoic acid hydroxylase, Cyp26a1, were also elevated. By immunohistochemical staining, TGF-β3 protein was found to be localized mainly in liver parenchymal cells (hepatocytes). These results indicate that retinoid mobilization likely takes place within the rat's liver following CCl4 treatment, and suggest the possibility that the expression of Tgfb mRNA is regulated by retinoic acid receptors. Reporter analyses of a region of the Tgfb3 gene were performed using the rat liver parenchymal cell line, RLC-16, and a positively responsive region was identified within its intron.

Journals

International Journal of Molecular Medicine

International Journal of Oncology

Molecular Medicine Reports

Oncology Reports

Experimental and Therapeutic Medicine

Oncology Letters

Biomedical Reports

Molecular and Clinical Oncology

World Academy of Sciences Journal

International Journal of Functional Nutrition

International Journal of Epigenetics

Medicine International

Elevated expression of transforming growth factor β3 in carbon tetrachloride-treated rat liver and involvement of retinoid signaling

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