Effect of the Hedgehog-inhibitor cyclopamine on mice with osteosarcoma pulmonary metastases

  • Authors:
    • Jörg Warzecha
    • Daniela Dinges
    • Balasz Kaszap
    • Dirk Henrich
    • Ingo Marzi
    • Caroline Seebach
  • View Affiliations

  • Published online on: December 1, 2011     https://doi.org/10.3892/ijmm.2011.851
  • Pages: 423-427
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Abstract

Chemoresistant metastases of osteosarcoma in humans limit survival in approximately one third of patients. Furthermore, aggressive chemotherapy can lead to side effects and occurrence of secondary malignancies in long time survivors. Therefore, supplemental medical strategies are worthwhile. The well-directed manipulation of cancer-signaling-cascades is an appealing approach. Targeting of the Hedgehog-pathway in cancer has led to promising results in vitro as well as in vivo in a number of different tumor types. Recently, the impact of cyclopamine, which inhibits Hedgehog signaling by binding to the receptor Smoothened, was shown in different human osteosarcoma cell lines in vitro and in vivo. In the present study we examined the influence of cyclopamine on early pulmonary metastases in vivo. Murine osteosarcoma cells, OS-50, were injected into the lateral tail vein of young BALB/c mice. Treatment with subcutaneous cyclopamine injections began after three days. Two weeks later, the animals were sacrificed and the number of pulmonary metastases was counted. We could observe a trend towards decreased metastases in the cyclopamine group (~20%). On the other hand, remarkable side effects were caused by the cyclopamine/ethanol/triolein preparation (mainly skin ulcerations).

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March 2012
Volume 29 Issue 3

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Spandidos Publications style
Warzecha J, Dinges D, Kaszap B, Henrich D, Marzi I and Seebach C: Effect of the Hedgehog-inhibitor cyclopamine on mice with osteosarcoma pulmonary metastases. Int J Mol Med 29: 423-427, 2012
APA
Warzecha, J., Dinges, D., Kaszap, B., Henrich, D., Marzi, I., & Seebach, C. (2012). Effect of the Hedgehog-inhibitor cyclopamine on mice with osteosarcoma pulmonary metastases. International Journal of Molecular Medicine, 29, 423-427. https://doi.org/10.3892/ijmm.2011.851
MLA
Warzecha, J., Dinges, D., Kaszap, B., Henrich, D., Marzi, I., Seebach, C."Effect of the Hedgehog-inhibitor cyclopamine on mice with osteosarcoma pulmonary metastases". International Journal of Molecular Medicine 29.3 (2012): 423-427.
Chicago
Warzecha, J., Dinges, D., Kaszap, B., Henrich, D., Marzi, I., Seebach, C."Effect of the Hedgehog-inhibitor cyclopamine on mice with osteosarcoma pulmonary metastases". International Journal of Molecular Medicine 29, no. 3 (2012): 423-427. https://doi.org/10.3892/ijmm.2011.851