[Corrigendum] A simplified 3D liver microsphere tissue culture model for hepatic cell signaling and drug-induced hepatotoxicity studies

• Authors:
  • Ying Zhu
  • Qiong Shi
  • Qi Peng
  • Yue Gao
  • Ting Yang
  • Yu Cheng
  • Hao Wang
  • Yetao Luo
  • Ailong Huang
  • Tong-Chuan He
  • Jiaming Fan

• View Affiliations

  
  Affiliations: Ministry of Education Key Laboratory of diagnostic Medicine, School of Laboratory Medicine, Chongqing Medical University, Chongqing 400016, P.R. China, department of Biostatistics, School of Public Health and Management, Chongqing Medical University, Chongqing 400016, P.R. China, Key Laboratory of Molecular Biology for Infectious diseases of The Ministry of Education of china, Institute for Viral Hepatitis, Department of Infectious Diseases, The Second Affiliated Hospital of Chongqing Medical University, Chongqing 400037, P.R. China, Molecular Oncology Laboratory, department of Orthopedic Surgery and Rehabilitation Medicine, The University of Chicago Medical center, Chicago, IL 60637, USA
• Published online on: March 6, 2023     https://doi.org/10.3892/ijmm.2023.5234
• Article Number: 31

• Copyright : © Zhu et al. This is an open access article distributed under the terms of Creative Commons Attribution License [CC BY 4.0].

Article

Int J Mol Med 44: [Related article:] 1653-1666, 2019; DOI: 10.3892/ijmm.2019.4321

Following the publication of the above article, an interested reader drew to the authors' attention that the 'Sift80, Day 7 / 10% FBS' data panel in Fig. 1Ba looked strikingly similar to the 'Sift80, 2% BCS / Day 3' data panel shown in Fig. 1Bb. After having re-examined their original data, the authors have realized that they inadvertently duplicated the data panel that correctly showed the results of the 'Sift80, Day 7 / 10% FBS' experiment in this figure.

Figure 1 Establishment of the 3-dimensional mouse liver microsphere tissue culture model. (A-a) Liver perfusion and isolation of mouse liver tissue. (b) Liver tissue was resected and rinsed with sterile PBS, followed by low speed centrifugation to remove blood cells. The recovered liver tissue was additionally minced and passed through 80-mesh (sift80) or 200-mesh (sift200) sift/strainers, and cultured in different concentrations of FBS or BCS/DMEM medium. (c) The recovered microsphere tissue exhibited significant auto-fluorescence, which was decreased by perfusion. (B-a) The morphology of the sift80 microsphere tissue cultured in 0, 2 or 10% FBS or BCS/DMEM at 7 days post-recovery, and (b) various sizes of liver tissue cultured in 2% BCS/DMEM at 0, 7 and 14 days after recovery. Each assay condition was performed in triplicate. Representative images are presented. BCS, bovine calf serum; DMEM, Dulbecco's modified Eagle's medium.

Therefore, the revised version of Fig. 1, now showing the correct data for the 'Sift80, 2% BCS / Day 3' panel, is shown on the next page. Note that the error made in assembling this figure did not affect the overall conclusions reported in the paper. All the authors agree with the publication of this corrigendum, and are grateful to the Editor of International Journal of Molecular Medicine for allowing them the opportunity to publish this. They also apologize to the readership for any inconvenience caused.

Figure 1 Establishment of the 3-dimensional mouse liver microsphere tissue culture model. (A-a) Liver perfusion and isolation of mouse liver tissue. (b) Liver tissue was resected and rinsed with sterile PBS, followed by low speed centrifugation to remove blood cells. The recovered liver tissue was additionally minced and passed through 80-mesh (sift80) or 200-mesh (sift200) sift/strainers, and cultured in different concentrations of FBS or BCS/DMEM medium. (c) The recovered microsphere tissue exhibited significant auto-fluorescence, which was decreased by perfusion. (B-a) The morphology of the sift80 microsphere tissue cultured in 0, 2 or 10% FBS or BCS/DMEM at 7 days post-recovery, and (b) various sizes of liver tissue cultured in 2% BCS/DMEM at 0, 7 and 14 days after recovery. Each assay condition was performed in triplicate. Representative images are presented. BCS, bovine calf serum; DMEM, Dulbecco's modified Eagle's medium.