Fourier transform infrared spectromicroscopy and hierarchical cluster analysis of human meningiomas

  • Authors:
    • Kaiser Ali
    • Yanjie Lu
    • Colleen Christensen
    • Tim May
    • Craig Hyett
    • Robert Griebel
    • Daryl Fourney
    • Kotoo Meguro
    • Lothar Resch
    • Rajendra K. Sharma
  • View Affiliations

  • Published online on: March 1, 2008     https://doi.org/10.3892/ijmm.21.3.297
  • Pages:297-301
0

Abstract

Limitations of conventional light microscopy in pathological diagnosis of brain tumors include subjective bias in interpretation and discordance of nomenclature. A study using mid-infrared (IR) spectromicroscopy was undertaken to determine whether meningiomas, a group of brain tumors prone to recurrence, could be identified by the unique spectral ‘fingerprints’ of their chemical composition. Paired, thin (5-μm) cryosections of snap-frozen human meningioma tumor samples removed at elective surgery were mounted on glass (hematoxylin and eosin-stained tissue section) and infrared (unstained tissue section) reflectance slides, respectively. Concordance of the tumor-bearing areas identified in the stained section by a pathologist with the unstained IR tissue section was ensured using a novel digital grid and tumor-mapping system developed in our laboratory. Compared with the normal control, tumor samples from four meningioma patients revealed a marked decrease in bands associated with unsaturated fatty acids, particularly in the bands at 3010, 2920, 2850, and 1735 cm−1. Spectral datasets were subjected to hierarchical cluster analyses (HCA) using Ward's algorithm for comparison and grouping of similar data groups, and were converted into color-coded digital maps for matching spectra with their respective clusters. False color images of 5 and 6 clusters obtained by HCA identified dominant clusters corresponding to tumor tissue. Corroboration of these findings in a larger number of meningiomas may allow for more precise identification of these and other types of brain tumors.

Related Articles

Journal Cover

March 2008
Volume 21 Issue 3

Print ISSN: 1107-3756
Online ISSN:1791-244X

2016 Impact Factor: 2.341
Ranked #21/128 Medicine Research and Experimental
(total number of cites)

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
APA
Ali, K., Lu, Y., Christensen, C., May, T., Hyett, C., Griebel, R. ... Sharma, R.K. (2008). Fourier transform infrared spectromicroscopy and hierarchical cluster analysis of human meningiomas. International Journal of Molecular Medicine, 21, 297-301. https://doi.org/10.3892/ijmm.21.3.297
MLA
Ali, K., Lu, Y., Christensen, C., May, T., Hyett, C., Griebel, R., Fourney, D., Meguro, K., Resch, L., Sharma, R. K."Fourier transform infrared spectromicroscopy and hierarchical cluster analysis of human meningiomas". International Journal of Molecular Medicine 21.3 (2008): 297-301.
Chicago
Ali, K., Lu, Y., Christensen, C., May, T., Hyett, C., Griebel, R., Fourney, D., Meguro, K., Resch, L., Sharma, R. K."Fourier transform infrared spectromicroscopy and hierarchical cluster analysis of human meningiomas". International Journal of Molecular Medicine 21, no. 3 (2008): 297-301. https://doi.org/10.3892/ijmm.21.3.297