TGF-β1 antisense therapy modulates expression of matrix metalloproteinases in keloid-derived fibroblasts

  • Authors:
    • H. Sadick
    • A. Herberger
    • K. Riedel
    • G. Bran
    • U. Goessler
    • K. Hoermann
    • F. Riedel
  • View Affiliations

  • Published online on: July 1, 2008     https://doi.org/10.3892/ijmm.22.1.55
  • Pages: 55-60
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Abstract

Transforming growth factor-β1 (TGF-β1) has been identified as an important regulator of wound healing. Recent developments in molecular therapy offer exciting prospects for the modulation of wound healing, specifically those targeting TGF-β1. The purpose of this study was to analyze the effect of TGF-β1 targeting on the expression of matrix metalloproteinases (MMPs) in fibroblasts cultured from earlobe keloids. The expression of MMP-2 and -9 in tissue samples from keloids was investigated by immunohistochemistry. The effect of TGF-β1 targeting using antisense oligonucleotides on the expression of MMPs in keloid-derived fibroblasts was analysed by ELISA and multiplex RT-PCR. Immunohistochemical studies demonstrated an increased expression of MMP protein in tissue samples from keloids compared to normal human skin. Antisense TGF-β1 oligonucleotide treatment significantly downregulated MMP-9 secretion in vitro. In conclusion, TGF-β1 antisense oligonucleotide technology may be a potential therapeutic option for the inhibition of proteolytic tissue destruction in keloids.

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July 2008
Volume 22 Issue 1

Print ISSN: 1107-3756
Online ISSN:1791-244X

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Spandidos Publications style
Sadick H, Herberger A, Riedel K, Bran G, Goessler U, Hoermann K and Riedel F: TGF-β1 antisense therapy modulates expression of matrix metalloproteinases in keloid-derived fibroblasts. Int J Mol Med 22: 55-60, 2008
APA
Sadick, H., Herberger, A., Riedel, K., Bran, G., Goessler, U., Hoermann, K., & Riedel, F. (2008). TGF-β1 antisense therapy modulates expression of matrix metalloproteinases in keloid-derived fibroblasts. International Journal of Molecular Medicine, 22, 55-60. https://doi.org/10.3892/ijmm.22.1.55
MLA
Sadick, H., Herberger, A., Riedel, K., Bran, G., Goessler, U., Hoermann, K., Riedel, F."TGF-β1 antisense therapy modulates expression of matrix metalloproteinases in keloid-derived fibroblasts". International Journal of Molecular Medicine 22.1 (2008): 55-60.
Chicago
Sadick, H., Herberger, A., Riedel, K., Bran, G., Goessler, U., Hoermann, K., Riedel, F."TGF-β1 antisense therapy modulates expression of matrix metalloproteinases in keloid-derived fibroblasts". International Journal of Molecular Medicine 22, no. 1 (2008): 55-60. https://doi.org/10.3892/ijmm.22.1.55