The role of the detection of hematogenous micrometastasis in prostate adenocarcinoma and malignant melanoma by RT-PCR.

  • Authors:
    • I Sardi
    • S Moretti
    • R Ponchietti
    • S Arrigucci
    • R Guazzelli
    • E Montali
  • View Affiliations

  • Published online on: April 1, 1999     https://doi.org/10.3892/ijmm.3.4.417
  • Pages: 417-426
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Abstract

Recent studies reported the possibility of detecting prostate adenocarcinoma and malignant melanoma cells in peripheral blood using RT-PCR of prostatic specific antigen (PSA), prostatic specific membrane antigen (PSMA) and Tyrosinase mRNAs. The PCR results showed high variability, ranging between 0% and 100% of positivity in patients with advanced disease. Our purpose was to evaluate the presence of tumor marker mRNAs in peripheral blood of prostate cancer and melanoma patients by means of RT-nested-PCR. We tested 70 and 36 peripheral blood samples from prostate carcinoma and malignant melanoma patients, respectively. The RT-PCR analysis showed the presence of PSA cDNA in 9 out of 70 (12.9%); PSMA cDNA in 14 out of 70 (20%); and Tyrosinase cDNA in 2 out of 36 (5.5%) peripheral blood samples from melanoma patients. Our study confirms the applicability of this sensitive method to monitor disease status. Although, the RT-nested-PCR of Tyrosinase is able to detect neoplastic cells in peripheral blood specimens, we suggest the necessity of a great caution in interpreting PCR results when the nested method has been used.

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Apr 1999
Volume 3 Issue 4

Print ISSN: 1107-3756
Online ISSN:1791-244X

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Spandidos Publications style
Sardi I, Moretti S, Ponchietti R, Arrigucci S, Guazzelli R and Montali E: The role of the detection of hematogenous micrometastasis in prostate adenocarcinoma and malignant melanoma by RT-PCR.. Int J Mol Med 3: 417-426, 1999
APA
Sardi, I., Moretti, S., Ponchietti, R., Arrigucci, S., Guazzelli, R., & Montali, E. (1999). The role of the detection of hematogenous micrometastasis in prostate adenocarcinoma and malignant melanoma by RT-PCR.. International Journal of Molecular Medicine, 3, 417-426. https://doi.org/10.3892/ijmm.3.4.417
MLA
Sardi, I., Moretti, S., Ponchietti, R., Arrigucci, S., Guazzelli, R., Montali, E."The role of the detection of hematogenous micrometastasis in prostate adenocarcinoma and malignant melanoma by RT-PCR.". International Journal of Molecular Medicine 3.4 (1999): 417-426.
Chicago
Sardi, I., Moretti, S., Ponchietti, R., Arrigucci, S., Guazzelli, R., Montali, E."The role of the detection of hematogenous micrometastasis in prostate adenocarcinoma and malignant melanoma by RT-PCR.". International Journal of Molecular Medicine 3, no. 4 (1999): 417-426. https://doi.org/10.3892/ijmm.3.4.417