Alpha-amylase functions as a salivary gland-specific self T cell epitope in patients with Sjögren's syndrome.

Matsumoto,I; Maeda,T; Takemoto,Y; Hashimoto,Y; Kimura,F; Iwamoto,I; Saito,Y; Nishioka,K; Sumida,T

doi:10.3892/ijmm.3.5.485

May 1999 Volume 3 Issue 5

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May 1999 Volume 3 Issue 5

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Article

Alpha-amylase functions as a salivary gland-specific self T cell epitope in patients with Sjögren's syndrome.

Authors:
- I Matsumoto
- T Maeda
- Y Takemoto
- Y Hashimoto
- F Kimura
- I Iwamoto
- Y Saito
- K Nishioka
- T Sumida
View Affiliations / Copyright

Affiliations: Division of Rheumatology, Immunology, and Genetic Program, Institute of Medical Science, St. Marianna University School of Medicine, Kawasaki, Japan.
Pages: 485-575
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Published online on: May 1, 1999

https://doi.org/10.3892/ijmm.3.5.485
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Abstract

Analyses of T cell receptors (TCR) on T cells infiltrating labial salivary glands of patients with Sjögren's syndrome (SS) indicate that the cells expand by antigen stimulation in context of major histocompatibility complex (MHC). To elucidate the autoantigens recognized by T cells infiltrating in labial salivary glands from patients with SS, proteins derived from human salivary gland cDNA libraries were screened by West-Western method using TCR-CDR3 probe, which is antigen recognition region of TCR on T cells. 13 cDNA clones were detected as proteins binding to TCR-CDR3 region. One was a human alpha-amylase salivary precursor (AA54-407), suggesting that alpha-amylase might be a salivary gland-specific autoantigen. To examine whether alpha-amylase acts as an antigen in labial salivary glands, PBL from 11 patients with SS were incubated with 9 different synthetic amino acids of alpha-amylase or salivary alpha-amylase. SSCP analysis on TCR clearly showed that alpha-amylase reactive T cells were observed in labial salivary glands from 3 of 11 patients with SS (27%). These findings support the possibility that alpha-amylase functions as a salivary gland-specific T cell epitope and induces autoimmunity in SS.

Journals

International Journal of Molecular Medicine

International Journal of Oncology

Molecular Medicine Reports

Oncology Reports

Experimental and Therapeutic Medicine

Oncology Letters

Biomedical Reports

Molecular and Clinical Oncology

World Academy of Sciences Journal

International Journal of Functional Nutrition

International Journal of Epigenetics

Medicine International

Alpha-amylase functions as a salivary gland-specific self T cell epitope in patients with Sjögren's syndrome.

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Abstract

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