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International Journal of Molecular Medicine
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Print ISSN: 1107-3756 Online ISSN: 1791-244X
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Mar 2000 Volume 5 Issue 3

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Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

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Mar 2000 Volume 5 Issue 3

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Article

Recombinant Semliki forest virus infects and kills human prostate cancer cell lines and prostatic duct epithelial cells ex vivo.

  • Authors:
    • P A Hardy
    • M J Mazzini
    • C Schweitzer
    • K Lundstrom
    • L M Glode
  • View Affiliations / Copyright

    Affiliations: Medical Oncology B171, School of Medicine, University of Colorado Health Sciences Center, Denver, CO 80262, USA.
  • Pages: 241-246
    |
    Published online on: March 1, 2000
       https://doi.org/10.3892/ijmm.5.3.241
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Abstract

Semliki Forest Virus (SFV) is a broad host range RNA virus capable of high-level recombinant protein expression and apoptosis induction in many cell types. We have successfully used a recombinant, replication deficient SFV vector to express the LacZ marker gene product in seven human prostate cell lines, as well as in human prostate tissue explants. Flow cytometry revealed that 40-60% of PPC-1 prostate cancer cells died 24-72 h after infection with SFV-LacZ virus. Most human prostate cancer cell lines expressed high levels of recombinant protein. Infection of human prostate tissue ex vivo led to similarly high expression levels but the recombinant beta-galactosidase was confined to duct epithelial cells. Infection of cell and tissue cultures resulted in detachment of adherent cells from the culture surface and detachment of epithelial cells from the basement membrane of tissue. Our results indicate that SFV may be useful in targeting recombinant protein expression and apoptosis to prostatic duct epithelial cells.

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Copy and paste a formatted citation
Spandidos Publications style
Hardy P, Mazzini M, Schweitzer C, Lundstrom K and Glode L: Recombinant Semliki forest virus infects and kills human prostate cancer cell lines and prostatic duct epithelial cells ex vivo.. Int J Mol Med 5: 241-246, 2000.
APA
Hardy, P., Mazzini, M., Schweitzer, C., Lundstrom, K., & Glode, L. (2000). Recombinant Semliki forest virus infects and kills human prostate cancer cell lines and prostatic duct epithelial cells ex vivo.. International Journal of Molecular Medicine, 5, 241-246. https://doi.org/10.3892/ijmm.5.3.241
MLA
Hardy, P., Mazzini, M., Schweitzer, C., Lundstrom, K., Glode, L."Recombinant Semliki forest virus infects and kills human prostate cancer cell lines and prostatic duct epithelial cells ex vivo.". International Journal of Molecular Medicine 5.3 (2000): 241-246.
Chicago
Hardy, P., Mazzini, M., Schweitzer, C., Lundstrom, K., Glode, L."Recombinant Semliki forest virus infects and kills human prostate cancer cell lines and prostatic duct epithelial cells ex vivo.". International Journal of Molecular Medicine 5, no. 3 (2000): 241-246. https://doi.org/10.3892/ijmm.5.3.241
Copy and paste a formatted citation
x
Spandidos Publications style
Hardy P, Mazzini M, Schweitzer C, Lundstrom K and Glode L: Recombinant Semliki forest virus infects and kills human prostate cancer cell lines and prostatic duct epithelial cells ex vivo.. Int J Mol Med 5: 241-246, 2000.
APA
Hardy, P., Mazzini, M., Schweitzer, C., Lundstrom, K., & Glode, L. (2000). Recombinant Semliki forest virus infects and kills human prostate cancer cell lines and prostatic duct epithelial cells ex vivo.. International Journal of Molecular Medicine, 5, 241-246. https://doi.org/10.3892/ijmm.5.3.241
MLA
Hardy, P., Mazzini, M., Schweitzer, C., Lundstrom, K., Glode, L."Recombinant Semliki forest virus infects and kills human prostate cancer cell lines and prostatic duct epithelial cells ex vivo.". International Journal of Molecular Medicine 5.3 (2000): 241-246.
Chicago
Hardy, P., Mazzini, M., Schweitzer, C., Lundstrom, K., Glode, L."Recombinant Semliki forest virus infects and kills human prostate cancer cell lines and prostatic duct epithelial cells ex vivo.". International Journal of Molecular Medicine 5, no. 3 (2000): 241-246. https://doi.org/10.3892/ijmm.5.3.241
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