Elongation factor-1alpha as a homologous complement activator of Jurkat cells.

  • Authors:
    • H Aoki
    • F Takizawa
    • S Tsuji
    • S Nagasawa
  • View Affiliations

  • Published online on: July 1, 2000     https://doi.org/10.3892/ijmm.6.1.87
  • Pages: 87-179
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Abstract

Although increasing evidence exist suggesting that apoptotic cells activate homologous complement, the homologous complement activators are only poorly characterized. We found that cell lysate of Jurkat cells contained a homologous complement activator of 50 kDa. Digestion of the 50 kDa activator with lysylendopeptidase yielded peptide fragments, with sequences identical to those of EF-1alpha. The 50 kDa activator was removed by immunoadsorption with anti-EF-1alpha, suggesting that the 50 kDa activator is EF-1alpha. The homologous complement activation did not proceed with EGTA-serum. In addition, C4b, a fragment produced by activation of the classical or lectin pathways was found to bind with EF-1alpha. These results suggest that EF-1alpha activates the homologous complement through the classical or lectin pathway.

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Jul 2000
Volume 6 Issue 1

Print ISSN: 1107-3756
Online ISSN:1791-244X

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Spandidos Publications style
Aoki H, Takizawa F, Tsuji S and Nagasawa S: Elongation factor-1alpha as a homologous complement activator of Jurkat cells.. Int J Mol Med 6: 87-179, 2000
APA
Aoki, H., Takizawa, F., Tsuji, S., & Nagasawa, S. (2000). Elongation factor-1alpha as a homologous complement activator of Jurkat cells.. International Journal of Molecular Medicine, 6, 87-179. https://doi.org/10.3892/ijmm.6.1.87
MLA
Aoki, H., Takizawa, F., Tsuji, S., Nagasawa, S."Elongation factor-1alpha as a homologous complement activator of Jurkat cells.". International Journal of Molecular Medicine 6.1 (2000): 87-179.
Chicago
Aoki, H., Takizawa, F., Tsuji, S., Nagasawa, S."Elongation factor-1alpha as a homologous complement activator of Jurkat cells.". International Journal of Molecular Medicine 6, no. 1 (2000): 87-179. https://doi.org/10.3892/ijmm.6.1.87