The gene of Ca2+-binding protein regucalcin is highly conserved in vertebrate species.

  • Authors:
    • H Misawa
    • M Yamaguchi
  • View Affiliations

  • Published online on: August 1, 2000     https://doi.org/10.3892/ijmm.6.2.191
  • Pages: 191-197
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Abstract

Regucalcin is a Ca2+-binding protein, which plays a regulatory role in liver cell functions related to Ca2+. In this study we have cloned cDNA for regucalcin from rabbit, bovine, chicken and toad livers by using rapid amplification of cDNA ends (RACE) method. The nucleotide and amino acid sequences of them are compared with published human, rat and mouse sequences. Comparison analysis revealed that the nucleotide sequences of regucalcin from seven vertebrate species were highly conserved in their coding region. The overall regucalcin proteins in these species consisted of 299 amino acids, and they had 69.9-91.3% identity. Furthermore, phylogenetic analysis showed that regucalcin in seven species appears to form a single cluster. This study demonstrates a great conservation of the regucalcin genes throughout evolution.

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Aug 2000
Volume 6 Issue 2

Print ISSN: 1107-3756
Online ISSN:1791-244X

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Spandidos Publications style
Misawa H and Yamaguchi M: The gene of Ca2+-binding protein regucalcin is highly conserved in vertebrate species.. Int J Mol Med 6: 191-197, 2000.
APA
Misawa, H., & Yamaguchi, M. (2000). The gene of Ca2+-binding protein regucalcin is highly conserved in vertebrate species.. International Journal of Molecular Medicine, 6, 191-197. https://doi.org/10.3892/ijmm.6.2.191
MLA
Misawa, H., Yamaguchi, M."The gene of Ca2+-binding protein regucalcin is highly conserved in vertebrate species.". International Journal of Molecular Medicine 6.2 (2000): 191-197.
Chicago
Misawa, H., Yamaguchi, M."The gene of Ca2+-binding protein regucalcin is highly conserved in vertebrate species.". International Journal of Molecular Medicine 6, no. 2 (2000): 191-197. https://doi.org/10.3892/ijmm.6.2.191