Activation of glycogen synthase a in hepatocytes exposed to alpha-D-glucose pentaacetate.

  • Authors:
    • J Cancelas
    • A Acitores
    • M L Villanueva-Penacarrillo
    • W J Malaisse
    • I Valverde
  • View Affiliations

  • Published online on: August 1, 2000     https://doi.org/10.3892/ijmm.6.2.197
  • Pages: 197-206
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Abstract

The effects of alpha-D-glucose pentaacetate (1.7 mM) upon glycogen synthase a activity and lactate output were examined in rat hepatocytes incubated at increasing concentrations of D-glucose. The ester enhanced the activity of glycogen synthase a at all concentrations (2.8, 4.0 and 8.0 mM) of D-glucose, which itself provoked a concentration-related increase in enzymatic activity. Likewise, the output of lactate augmented at increasing concentrations of D-glucose. However, alpha-D-glucose pentaacetate failed to cause a further increase in lactate output, the trend being even towards a lower production of lactate in the presence than absence of the ester. These findings suggest that the activation of glycogen synthase a by alpha-D-glucose pentaacetate and the subsequent increase in glycogen synthesis are sufficiently pronounced to prevent the increase in glycolysis otherwise expected from the generation of unesterified D-glucose from the same ester. Such a situation, which differs from that previously documented in pancreatic islet cells, could be favourable in the perspective of using alpha-D-glucose pentaacetate as a novel insulinotropic, and hence hypoglycaemic, tool in the treatment of non-insulin-dependent diabetes mellitus.

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Aug 2000
Volume 6 Issue 2

Print ISSN: 1107-3756
Online ISSN:1791-244X

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Spandidos Publications style
Cancelas J, Acitores A, Villanueva-Penacarrillo M, Malaisse W and Valverde I: Activation of glycogen synthase a in hepatocytes exposed to alpha-D-glucose pentaacetate.. Int J Mol Med 6: 197-206, 2000
APA
Cancelas, J., Acitores, A., Villanueva-Penacarrillo, M., Malaisse, W., & Valverde, I. (2000). Activation of glycogen synthase a in hepatocytes exposed to alpha-D-glucose pentaacetate.. International Journal of Molecular Medicine, 6, 197-206. https://doi.org/10.3892/ijmm.6.2.197
MLA
Cancelas, J., Acitores, A., Villanueva-Penacarrillo, M., Malaisse, W., Valverde, I."Activation of glycogen synthase a in hepatocytes exposed to alpha-D-glucose pentaacetate.". International Journal of Molecular Medicine 6.2 (2000): 197-206.
Chicago
Cancelas, J., Acitores, A., Villanueva-Penacarrillo, M., Malaisse, W., Valverde, I."Activation of glycogen synthase a in hepatocytes exposed to alpha-D-glucose pentaacetate.". International Journal of Molecular Medicine 6, no. 2 (2000): 197-206. https://doi.org/10.3892/ijmm.6.2.197