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International Journal of Molecular Medicine
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Print ISSN: 1107-3756 Online ISSN: 1791-244X
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October 2001 Volume 8 Issue 4

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International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

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International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

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Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

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Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

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Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

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Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

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International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

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Article

Dimerization of small GTPase Rab5

  • Authors:
    • H. Daitoku
    • J. Isida
    • K. Fujiwara
    • T. Nakajima
    • A. Fukamizu

  • View Affiliations / Copyright

    Affiliations: Institute of Applied Biochemistry, University of Tsukuba, Tsukuba, Ibaraki, Japan
  • Pages: 397-404
    |
    Published online on: October 1, 2001
       https://doi.org/10.3892/ijmm.8.4.397
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Abstract

Rab proteins are small GTPases, localized to distinct cellular compartments, regulating specific steps of intracellular membrane trafficking. One member of the Rab family, Rab5, consists of three isoforms, Rab5a, Rab5b, and Rab5c, which have been shown to play an important role in early events of endocytosis. Using the yeast two-hybrid system, we have identified several cytosolic proteins that interact with the Rab5b Q79L (decreasing intrinsic and GTPase-activating protein-stimulated GTPase activities). Surprisingly, most positive clones were Rab5b or Rab5c, indicating that Rab5 could dimerize among extra-isoforms in the yeast two-hybrid system. In vitro and in vivo chemical cross-linking assays demonstrated that lipid-unmodified wild-type Rab5b purified from Escherichia coli, wild-type Rab5b, or a dominant active form Rab5b Q79L expressed in human 293T cells dimerized. Furthermore, the same assays using a Rab5b R81A substitution mutant showed that the Arg81 in the Switch II region [the second GTP/GDP binding motif (residues 74-93)] was essential for Rab5b dimerization. These results suggest that Rab5 isoforms can be dimerized depending upon the GTP-bound conformation, but independent upon a lipid modification.

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Copy and paste a formatted citation
Spandidos Publications style
Daitoku H, Isida J, Fujiwara K, Nakajima T and Fukamizu A: Dimerization of small GTPase Rab5. Int J Mol Med 8: 397-404, 2001.
APA
Daitoku, H., Isida, J., Fujiwara, K., Nakajima, T., & Fukamizu, A. (2001). Dimerization of small GTPase Rab5. International Journal of Molecular Medicine, 8, 397-404. https://doi.org/10.3892/ijmm.8.4.397
MLA
Daitoku, H., Isida, J., Fujiwara, K., Nakajima, T., Fukamizu, A."Dimerization of small GTPase Rab5". International Journal of Molecular Medicine 8.4 (2001): 397-404.
Chicago
Daitoku, H., Isida, J., Fujiwara, K., Nakajima, T., Fukamizu, A."Dimerization of small GTPase Rab5". International Journal of Molecular Medicine 8, no. 4 (2001): 397-404. https://doi.org/10.3892/ijmm.8.4.397
Copy and paste a formatted citation
x
Spandidos Publications style
Daitoku H, Isida J, Fujiwara K, Nakajima T and Fukamizu A: Dimerization of small GTPase Rab5. Int J Mol Med 8: 397-404, 2001.
APA
Daitoku, H., Isida, J., Fujiwara, K., Nakajima, T., & Fukamizu, A. (2001). Dimerization of small GTPase Rab5. International Journal of Molecular Medicine, 8, 397-404. https://doi.org/10.3892/ijmm.8.4.397
MLA
Daitoku, H., Isida, J., Fujiwara, K., Nakajima, T., Fukamizu, A."Dimerization of small GTPase Rab5". International Journal of Molecular Medicine 8.4 (2001): 397-404.
Chicago
Daitoku, H., Isida, J., Fujiwara, K., Nakajima, T., Fukamizu, A."Dimerization of small GTPase Rab5". International Journal of Molecular Medicine 8, no. 4 (2001): 397-404. https://doi.org/10.3892/ijmm.8.4.397
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