An anti-GD2 single chain Fv selected by phage display and fused to Pseudomonas exotoxin A develops specific cytotoxic activity against neuroblastoma derived cell lines

  • Authors:
    • M. K. Tur
    • S. Sasse
    • M. Stocker
    • K. Djabelkhir
    • M. Huhn
    • B. Matthey
    • C. Gottstein
    • T. Pfitzner
    • A. Engert
    • S. Barth
  • View Affiliations

  • Published online on: November 1, 2001     https://doi.org/10.3892/ijmm.8.5.579
  • Pages: 579-584
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

Since the disialoganglioside GD2 is abundantly present on the surface of neuroblastoma cells, we constructed a new recombinant immunotoxin for possible clinical use in patients with neuroblastoma. A functional 14.18 scFv-phage was obtained by selection of an anti-GD2 hybridoma derived phage antibody mini-library on the neuroblastoma-derived, GD2-expressing cell line IMR5. By insertion into the bacterial expression vector pBM1.1 the selected scFv was fused to a deletion mutant of Pseudomonas exotoxin A (ETA'). Periplasmically expressed 14.18(scFv)-ETA' bound to the GD2 expressing cell line IMR5, but not to the GD2 negative Hodgkin-derived cell line L540Cy as documented by ELISA and flow cytometry. The recombinant immunotoxin (rIT) inhibited cell viability of IMR5 cells by 50% at concentrations (IC50) of 0.326 μg/ml. This recombinant immunotoxin will be further investigated in vivo for its value as a new immunotherapeutic agent for the treatment of patients with neuroblastoma.

Related Articles

Journal Cover

November 2001
Volume 8 Issue 5

Print ISSN: 1107-3756
Online ISSN:1791-244X

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
x
Spandidos Publications style
Tur MK, Sasse S, Stocker M, Djabelkhir K, Huhn M, Matthey B, Gottstein C, Pfitzner T, Engert A, Barth S, Barth S, et al: An anti-GD2 single chain Fv selected by phage display and fused to Pseudomonas exotoxin A develops specific cytotoxic activity against neuroblastoma derived cell lines. Int J Mol Med 8: 579-584, 2001
APA
Tur, M.K., Sasse, S., Stocker, M., Djabelkhir, K., Huhn, M., Matthey, B. ... Barth, S. (2001). An anti-GD2 single chain Fv selected by phage display and fused to Pseudomonas exotoxin A develops specific cytotoxic activity against neuroblastoma derived cell lines. International Journal of Molecular Medicine, 8, 579-584. https://doi.org/10.3892/ijmm.8.5.579
MLA
Tur, M. K., Sasse, S., Stocker, M., Djabelkhir, K., Huhn, M., Matthey, B., Gottstein, C., Pfitzner, T., Engert, A., Barth, S."An anti-GD2 single chain Fv selected by phage display and fused to Pseudomonas exotoxin A develops specific cytotoxic activity against neuroblastoma derived cell lines". International Journal of Molecular Medicine 8.5 (2001): 579-584.
Chicago
Tur, M. K., Sasse, S., Stocker, M., Djabelkhir, K., Huhn, M., Matthey, B., Gottstein, C., Pfitzner, T., Engert, A., Barth, S."An anti-GD2 single chain Fv selected by phage display and fused to Pseudomonas exotoxin A develops specific cytotoxic activity against neuroblastoma derived cell lines". International Journal of Molecular Medicine 8, no. 5 (2001): 579-584. https://doi.org/10.3892/ijmm.8.5.579