Expression and distribution of cyclooxygenase-2 in human periovulatory ovary

  • Authors:
    • O. Tokuyama
    • Y. Nakamura
    • A. Muso
    • K.-I. Honda
    • O. Ishiko
    • S. Ogita
  • View Affiliations

  • Published online on: December 1, 2001     https://doi.org/10.3892/ijmm.8.6.603
  • Pages: 603-606
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Abstract

Cyclooxygenase, which converts arachidonic acid into prostaglandins, has two types of isoforms, cyclooxygenase-1 and cyclooxygenase-2 (COX-2). The latter is thought to be essential for the ovulatory mechanism. However, expression or distribution of COX-2 in periovulatory human ovary has not been reported. The aim of our study was to examine COX-2 expression and distribution in pre- and postovulatory human ovary. COX-2 was detected by Western blot analysis of pre-ovulatory human ovarian follicular fluid. The levels of COX-2 in preovulatory fluid obtained from 20 subjects underwent in vitro fertilization and embryo transfer, and were assayed using enzyme immunoassay for human COX-2. The results showed 7.3±4.1 ng/ml (mean ± SD) with a COX-2 level range of 2.7 to 19.5 ng/ml of follicular fluid. Detection of COX-2 is considered to reflect its production in the preovulatory follicle. Immunofluorescence microscopic examination of an ovary obtained from a woman at the postovulatory period showed distribution of COX-2 in interstitial but not in granulosa cells in a ruptured follicle. These findings collectively suggest the possibility that COX-2 is mainly produced in follicles in a preovulatory phase, while after ovulation, COX-2 is produced in interstitial cells in human ovary.

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December 2001
Volume 8 Issue 6

Print ISSN: 1107-3756
Online ISSN:1791-244X

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APA
Tokuyama, O., Nakamura, Y., Muso, A., Honda, K., Ishiko, O., & Ogita, S. (2001). Expression and distribution of cyclooxygenase-2 in human periovulatory ovary. International Journal of Molecular Medicine, 8, 603-606. https://doi.org/10.3892/ijmm.8.6.603
MLA
Tokuyama, O., Nakamura, Y., Muso, A., Honda, K., Ishiko, O., Ogita, S."Expression and distribution of cyclooxygenase-2 in human periovulatory ovary". International Journal of Molecular Medicine 8.6 (2001): 603-606.
Chicago
Tokuyama, O., Nakamura, Y., Muso, A., Honda, K., Ishiko, O., Ogita, S."Expression and distribution of cyclooxygenase-2 in human periovulatory ovary". International Journal of Molecular Medicine 8, no. 6 (2001): 603-606. https://doi.org/10.3892/ijmm.8.6.603