Stimulatory effect of zinc on insulin-like growth factor-I and transforming growth factor-β1 production with bone growth of newborn rats

Ma,Z. J.; Misawa,H.; Yamaguchi,M.

doi:10.3892/ijmm.8.6.623

December 2001 Volume 8 Issue 6

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December 2001 Volume 8 Issue 6

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Article

Stimulatory effect of zinc on insulin-like growth factor-I and transforming growth factor-β1 production with bone growth of newborn rats

Authors:
- Z. J. Ma
- H. Misawa
- M. Yamaguchi
View Affiliations / Copyright

Affiliations: Laboratory of Endocrinology and Molecular Metabolism, Graduate School of Nutritional Sciences, University of Shizuoka, Shizuoka 422-8526, Japan
Pages: 623-628
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Published online on: December 1, 2001

https://doi.org/10.3892/ijmm.8.6.623
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Abstract

The effect of zinc, an essential trace element, on insulin-like growth factor-I (IGF-I) and transforming growth factor-β1 production was investigated to determine the role of this metal in bone growth of newborn rats. Femoral-diaphyseal and metaphyseal tissues were obtained between 1 and 28 days after birth of newborn rats, and cultured for 24 h in a serum-free Dulbecco's modified Eagle's medium containing either vehicle or zinc sulfate (10-6-10-4 M). Protein concentration in the medium was significantly increased by culture with bone tissues of newborn rats with increasing age (14 and 21 days). Medium IGF-I and TGF-β1 concentration was gradually reduced with increasing age after birth. The presence of zinc (10-5 and 10-4 M) caused a significant increase in protein, IGF-I, and TGF-β1 concentrations in the medium cultured with the diaphyseal or metaphyseal tissues obtained at 7 and 14 days after birth. The expression of IGF-I and TGF-β1 mRNA was demonstrated by reverse transcription-polymerase chain reaction (RT-PCR) analysis in the diaphyseal and metaphyseal tissues cultured for 24 h using rat IGF-I or TGF-β1-specific primers. These expressions were significantly raised in the presence of zinc (10-4 M) in culture medium. The present study demonstrates that zinc has a stimulatory effect on IGF-I and TGF-β1 production in the femoral tissues with bone growth of newborn rats.

Journals

International Journal of Molecular Medicine

International Journal of Oncology

Molecular Medicine Reports

Oncology Reports

Experimental and Therapeutic Medicine

Oncology Letters

Biomedical Reports

Molecular and Clinical Oncology

World Academy of Sciences Journal

International Journal of Functional Nutrition

International Journal of Epigenetics

Medicine International

Stimulatory effect of zinc on insulin-like growth factor-I and transforming growth factor-β1 production with bone growth of newborn rats

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