T cell dependent and independent antitumor immunity generated by the expression of Fas ligand on mouse lung carcinoma cells

  • Authors:
    • Yuji Tada
    • Jiyang O-Wang
    • Yuichi Takiguchi
    • Koichiro Tatsumi
    • Takayuki Kuriyama
    • Masatoshi Tagawa
  • View Affiliations

  • Published online on: March 1, 2002     https://doi.org/10.3892/ijmm.9.3.281
  • Pages: 281-285
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

Interaction between Fas and Fas ligand (FasL) induces apoptotic cell death of Fas-positive cells. Expression of FasL on tumors therefore possibly kills activated Fas-positive cytotoxic T cells that infiltrated into the tumors and consequently the tumors can evade from systemic immune responses. Previous studies however showed that forced expression of FasL in tumors induced neutrophil-mediated inflammatory reactions and accordingly produced T cell independent antitumor effects in the inoculated animals. We then analyzed the FasL-mediated antitumor responses with genetically mutated mice. Murine lung carcinoma (A11) cells transfected with the FasL gene (A11/FasL), which was able to kill Fas-positive B cells, did not form subcutaneous tumors and produced few lung spontaneous metastatic foci in immunocompetent mice. The mice that rejected A11/FasL cells developed tumor-specific protective immunity. A11/FasL cells were also rejected in T cell-defective nude mice and in CD18-defecient mice which showed impaired neutrophil functions, but not in Fas-defective (lpr/lpr) mutant mice. Antitumor activities on A11 cells were dependent on the number of coinjected A11/FasL cells but those on irrelevant B16 murine melanoma cells were not produced even with a large number of coinjected A11/FasL cells. In contrast to previous reports, the present study implies that T cells can also be effectors of FasL-mediated antitumor responses and neutrophils are not absolutely required for the responses.

Related Articles

Journal Cover

March 2002
Volume 9 Issue 3

Print ISSN: 1107-3756
Online ISSN:1791-244X

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
x
Spandidos Publications style
Tada Y, O-Wang J, Takiguchi Y, Tatsumi K, Kuriyama T and Tagawa M: T cell dependent and independent antitumor immunity generated by the expression of Fas ligand on mouse lung carcinoma cells. Int J Mol Med 9: 281-285, 2002
APA
Tada, Y., O-Wang, J., Takiguchi, Y., Tatsumi, K., Kuriyama, T., & Tagawa, M. (2002). T cell dependent and independent antitumor immunity generated by the expression of Fas ligand on mouse lung carcinoma cells. International Journal of Molecular Medicine, 9, 281-285. https://doi.org/10.3892/ijmm.9.3.281
MLA
Tada, Y., O-Wang, J., Takiguchi, Y., Tatsumi, K., Kuriyama, T., Tagawa, M."T cell dependent and independent antitumor immunity generated by the expression of Fas ligand on mouse lung carcinoma cells". International Journal of Molecular Medicine 9.3 (2002): 281-285.
Chicago
Tada, Y., O-Wang, J., Takiguchi, Y., Tatsumi, K., Kuriyama, T., Tagawa, M."T cell dependent and independent antitumor immunity generated by the expression of Fas ligand on mouse lung carcinoma cells". International Journal of Molecular Medicine 9, no. 3 (2002): 281-285. https://doi.org/10.3892/ijmm.9.3.281