Open Access

Circadian rhythm disorder of thrombosis and thrombolysis-related gene expression in apolipoprotein E knock-out mice

  • Authors:
    • Xueqing Zhang
    • Fengdi Zhao
    • Chen Xu
    • Chao Lu
    • Huiming Jin
    • Sifeng Chen
    • Ruizhe Qian
  • View Affiliations

  • Published online on: August 1, 2008     https://doi.org/10.3892/ijmm_00000002
  • Pages: 149-153
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Abstract

Plaque rupture and subsequent embolism as well as thrombosis are major causes of acute myocardial infarction and stroke secondary to atherosclerosis. Pai-1, t-PA, TF and ET-1 are thrombosis- and thrombolysis-related factors which play important roles in thrombosis formation and plaque rupture. Since acute myocardial infarction and stroke are more likely to occur between 6 a.m. and 12 p.m. than at another time of the day, we studied the relationship between circadian rhythm and Pai-1, t-PA, TF and ET-1 in normal and atherosclerotic mice. Atherosclerosis was developed in apoE−/− mice fed a normal diet or a high cholesterol diet. The expression of Pai-1, t-PA, TF and ET-1 in the hearts of control C57BL/6J mice and atherosclerotic mice was measured by real-time RT-PCR at different Zeitgeber times (ZT) including ZT0, ZT4, ZT8, ZT10, ZT12, ZT14, ZT16 and ZT20. The expression of Pai-1, t-PA, TF and ET-1 peaked between ZT14 and ZT16 and bottomed at ZT10 in C57BL/6J mice. Their expression in apoE−/− mice fed a normal diet lost circadian rhythm. Their expression in apoE−/− mice fed a high cholesterol diet peaked at ZT4, indicating a reverse circadian rhythm. Our result indicates that circadian changes in the expression of Pai-1, t-PA, TF and ET-1 may be involved in the onset of myocardial infarction and stroke.

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August 2008
Volume 22 Issue 2

Print ISSN: 1107-3756
Online ISSN:1791-244X

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Spandidos Publications style
Zhang X, Zhao F, Xu C, Lu C, Jin H, Chen S and Qian R: Circadian rhythm disorder of thrombosis and thrombolysis-related gene expression in apolipoprotein E knock-out mice. Int J Mol Med 22: 149-153, 2008
APA
Zhang, X., Zhao, F., Xu, C., Lu, C., Jin, H., Chen, S., & Qian, R. (2008). Circadian rhythm disorder of thrombosis and thrombolysis-related gene expression in apolipoprotein E knock-out mice. International Journal of Molecular Medicine, 22, 149-153. https://doi.org/10.3892/ijmm_00000002
MLA
Zhang, X., Zhao, F., Xu, C., Lu, C., Jin, H., Chen, S., Qian, R."Circadian rhythm disorder of thrombosis and thrombolysis-related gene expression in apolipoprotein E knock-out mice". International Journal of Molecular Medicine 22.2 (2008): 149-153.
Chicago
Zhang, X., Zhao, F., Xu, C., Lu, C., Jin, H., Chen, S., Qian, R."Circadian rhythm disorder of thrombosis and thrombolysis-related gene expression in apolipoprotein E knock-out mice". International Journal of Molecular Medicine 22, no. 2 (2008): 149-153. https://doi.org/10.3892/ijmm_00000002