Effect of vascular cell adhesion molecule 1 on fibroblasts in human eosinophilic chronic rhinosinusitis

  • Authors:
    • Alexander Sauter
    • Daniel Bas-Orth
    • Jens Stern-Straeter
    • James Barnes
    • Hannah Soulsby
    • Karl Hörmann
    • Ramin Naim
  • View Affiliations

  • Published online on: October 1, 2008     https://doi.org/10.3892/ijmm_00000040
  • Pages: 441-446
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Abstract

The pathogenesis of eosinophilic chronic rhinosinusitis (ECRS) is still unclear. Paranasal mucosa inflammation is thought to be related to eosinophilic infiltration. This infiltration seems to induce changes in the expression of cell adhesion molecules such as vascular cell adhesion molecule 1 (VCAM-1). The E-cadherin-β-catenin complex maintains the integrity of the epithelium. Downregulation of β-catenin and E-cadherin is a pivotal factor for progressive cell growth. This study aimed to assess which cytokines regulate the expression of the adhesion molecule E-cadherin and the multi-functional protein β-catenin, which plays a key role in cadherin-mediated anchoring in ECRS. Cultured ECRS specimens were incubated with human VCAM-1. After a period of up to 72 h, expression of E-cadherin and β-catenin was determined using cytokine immunoassay and immunohistochemistry. In ECRS, significant increases in E-cadherin expression were found in fibroblast cell cultures. Stimulation with VCAM-1 did not produce a significant alteration in the expression of the adherens junction protein β-catenin. In addition, VCAM-1 did not decrease the levels of membrane staining for adherens junction proteins. The selective increase in E-cadherin expression in eosinophilic fibroblast cultures might be explained by a higher concentration of the Th2-type cytokines in these cultures. The tissue remodelling observed during chronic eosinophilic inflammation offers new insight into the pathogenesis of ECRS.

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October 2008
Volume 22 Issue 4

Print ISSN: 1107-3756
Online ISSN:1791-244X

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Spandidos Publications style
Sauter A, Bas-Orth D, Stern-Straeter J, Barnes J, Soulsby H, Hörmann K and Naim R: Effect of vascular cell adhesion molecule 1 on fibroblasts in human eosinophilic chronic rhinosinusitis. Int J Mol Med 22: 441-446, 2008
APA
Sauter, A., Bas-Orth, D., Stern-Straeter, J., Barnes, J., Soulsby, H., Hörmann, K., & Naim, R. (2008). Effect of vascular cell adhesion molecule 1 on fibroblasts in human eosinophilic chronic rhinosinusitis. International Journal of Molecular Medicine, 22, 441-446. https://doi.org/10.3892/ijmm_00000040
MLA
Sauter, A., Bas-Orth, D., Stern-Straeter, J., Barnes, J., Soulsby, H., Hörmann, K., Naim, R."Effect of vascular cell adhesion molecule 1 on fibroblasts in human eosinophilic chronic rhinosinusitis". International Journal of Molecular Medicine 22.4 (2008): 441-446.
Chicago
Sauter, A., Bas-Orth, D., Stern-Straeter, J., Barnes, J., Soulsby, H., Hörmann, K., Naim, R."Effect of vascular cell adhesion molecule 1 on fibroblasts in human eosinophilic chronic rhinosinusitis". International Journal of Molecular Medicine 22, no. 4 (2008): 441-446. https://doi.org/10.3892/ijmm_00000040