Glucosamine, a naturally occurring amino monosaccharide modulates LL-37-induced endothelial cell activation

  • Authors:
    • Yinghua Ju
    • Jian Hua
    • Koji Sakamoto
    • Hideoki Ogawa
    • Isao Nagaoka
  • View Affiliations

  • Published online on: November 1, 2008     https://doi.org/10.3892/ijmm_00000069
  • Pages: 657-662
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

Atheroscleros is now considered as a chronic inflammatory disease, and glucosamine has a potential to exhibit anti-inflammatory action. Thus, we investigated the effect of glucosamine on LL-37-induced endothelial cell activation. HUVEC (human umbilical vein endothelial cells) were stimulated by LL-37 in the presence or absence of glucosamine (0.01-1 mM) or its analogue, N-acetylglucosamine (0.1-1 mM). mRNA expression of MCP-1 (monocyte chemoattractant protein-1) and ICAM-1 (intercellular adhesion molecule-1) was evaluated by real-time RT-PCR, and their protein levels were analyzed by ELISA and Western blotting, respectively. Furthermore, the effect of glucosamine on O-N-acetylglucosamine (O-GlcNAc) modification was evaluated by Western blotting. Glucosamine but not N-acetylglucosamine suppressed the LL-37-induced expression of MCP-1 and ICAM-1 at both mRNA (p<0.05 at 0.1 mM) and protein levels (p<0.05 at 1 mM). Of interest, O-GlcNAc modification was induced by incubating HUVEC with glucosamine (p<0.05 at 1 mM) but not N-acetylglucosamine. Of note, alloxan, an O-N-acetylglucosamine transferase inhibitor, which prevented the glucosamine-induced O-GlcNAc modification, abrogated the suppressive effect of glucosamine on MCP-1 and ICAM-1 expression (p<0.05 at 0.5 mM). These observations suggest that glucosamine modulates endothelial cell activation possibly via O-GlcNAc modification, and may exhibit an anti-inflammatory action on atherosclerosis.

Related Articles

Journal Cover

November 2008
Volume 22 Issue 5

Print ISSN: 1107-3756
Online ISSN:1791-244X

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
x
Spandidos Publications style
Ju Y, Hua J, Sakamoto K, Ogawa H and Nagaoka I: Glucosamine, a naturally occurring amino monosaccharide modulates LL-37-induced endothelial cell activation. Int J Mol Med 22: 657-662, 2008
APA
Ju, Y., Hua, J., Sakamoto, K., Ogawa, H., & Nagaoka, I. (2008). Glucosamine, a naturally occurring amino monosaccharide modulates LL-37-induced endothelial cell activation. International Journal of Molecular Medicine, 22, 657-662. https://doi.org/10.3892/ijmm_00000069
MLA
Ju, Y., Hua, J., Sakamoto, K., Ogawa, H., Nagaoka, I."Glucosamine, a naturally occurring amino monosaccharide modulates LL-37-induced endothelial cell activation". International Journal of Molecular Medicine 22.5 (2008): 657-662.
Chicago
Ju, Y., Hua, J., Sakamoto, K., Ogawa, H., Nagaoka, I."Glucosamine, a naturally occurring amino monosaccharide modulates LL-37-induced endothelial cell activation". International Journal of Molecular Medicine 22, no. 5 (2008): 657-662. https://doi.org/10.3892/ijmm_00000069