Silibinin attenuates adipogenesis in 3T3-L1 preadipocytes through a potential upregulation of the insig pathway

  • Authors:
    • Sun-O Ka
    • Kyung-Ah Kim
    • Kang-Beom Kwon
    • Jin-Woo Park
    • Byung-Hyun Park
  • View Affiliations

  • Published online on: May 1, 2009     https://doi.org/10.3892/ijmm_00000174
  • Pages: 633-637
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Abstract

In the past few decades, the use of silibinin, a plant flavonoid extracted from the milk thistle, as a hepatoprotective and chemopreventive agent has gained much attention. In this study, we investigated the effects of silibinin on adipogenesis. Treatment with silibinin suppressed terminal differentiation of 3T3-L1 cells into adipocytes as evidenced by Oil red O staining and TG assay results. Real-time RT-PCR analysis revealed that silibinin decreased the expression of adipogenesis-related genes such as CAAT/enhancer binding protein-α, fatty acid synthase, sterol response element binding protein 1c, adipocyte-specific lipid binding protein, peroxisome proliferator-activated receptor γ and lipoprotein lipase, and increased the expression of preadipocyte factor-1, a preadipocyte marker gene. The anti-adipogenic effect of silibinin was associated with the up-regulation of insig-1 and insig-2. Collectively, these results suggest that silibinin inhibits adipocyte differentiation through a potential up-regulation of insig-1 and insig-2 at an early phase in adipocyte differentiation.

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May 2009
Volume 23 Issue 5

Print ISSN: 1107-3756
Online ISSN:1791-244X

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APA
Ka, S., Kim, K., Kwon, K., Park, J., & Park, B. (2009). Silibinin attenuates adipogenesis in 3T3-L1 preadipocytes through a potential upregulation of the insig pathway . International Journal of Molecular Medicine, 23, 633-637. https://doi.org/10.3892/ijmm_00000174
MLA
Ka, S., Kim, K., Kwon, K., Park, J., Park, B."Silibinin attenuates adipogenesis in 3T3-L1 preadipocytes through a potential upregulation of the insig pathway ". International Journal of Molecular Medicine 23.5 (2009): 633-637.
Chicago
Ka, S., Kim, K., Kwon, K., Park, J., Park, B."Silibinin attenuates adipogenesis in 3T3-L1 preadipocytes through a potential upregulation of the insig pathway ". International Journal of Molecular Medicine 23, no. 5 (2009): 633-637. https://doi.org/10.3892/ijmm_00000174