Attenuation of the oxidative burst-induced DNA damage in human leukocytes by hyaluronan

Halicka,H. Dorota; Mitlitski,Vadim; Heeter,Julie; Balazs,Endre A.; Darzynkiewicz,Zbigniew

doi:10.3892/ijmm_00000182

May 2009 Volume 23 Issue 5

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May 2009 Volume 23 Issue 5

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Article

Attenuation of the oxidative burst-induced DNA damage in human leukocytes by hyaluronan

Authors:
- H. Dorota Halicka
- Vadim Mitlitski
- Julie Heeter
- Endre A. Balazs
- Zbigniew Darzynkiewicz
View Affiliations / Copyright

Affiliations: Brander Cancer Research Institute and Department of Pathology, New York Medical College, Valhalla, NY, 10595, USA
Pages: 695-699
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Published online on: May 1, 2009

https://doi.org/10.3892/ijmm_00000182
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Abstract

Oxidative burst provides the mechanism for specialized phagocytes, such as granulocytes or monocytes, to kill invading microorganisms through generation of superoxide anions. However, the oxidants generated during the burst damage DNA of the phagocytes and neighboring cells. Human blood leukocytes treated with phorbol myristate acetate (PMA) are considered to represent the experimental model of induction of oxidative burst. We recently reported that DNA damage in PMA-treated leukocytes is assessed by cytometric analysis of the induction of histone H2AX phosphorylation and Ataxia Telangiectasia Mutated (ATM) activation. In the present study we observed that hyaluronic acid (HA) of average molecular weight (MW) 5.4x106 and 2x106 at 0.1% (w/v) concentration significantly attenuated H2AX phosphorylation and ATM activation induced in leukocytes during oxidative burst. HA also reduced the intracellular level of PMA-induced reactive oxidants as measured by the ability of cells to oxidize 2',7'-dihydro-dichlorofluorescein-diacetate. No such effect was seen with HA of 6x104 MW. The data are consistent with earlier observations that HA of high MW protects DNA from oxidative damage induced by endo- or exogenous oxidants. The anti-oxidant effect of HA seen during oxidative burst also explains its anti-inflammatory effect when used to treat arthritic joints.

Journals

International Journal of Molecular Medicine

International Journal of Oncology

Molecular Medicine Reports

Oncology Reports

Experimental and Therapeutic Medicine

Oncology Letters

Biomedical Reports

Molecular and Clinical Oncology

World Academy of Sciences Journal

International Journal of Functional Nutrition

International Journal of Epigenetics

Medicine International

Attenuation of the oxidative burst-induced DNA damage in human leukocytes by hyaluronan

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