Suberoylanilide hydroxamic acid (SAHA) changes microRNA expression profiles in A549 human non-small cell lung cancer cells

  • Authors:
    • Eun-Mee Lee
    • Sangsu Shin
    • Hwa Jun Cha
    • Youngmin Yoon
    • Seunghee Bae
    • Jin Hyuk Jung
    • Sun-Mi Lee
    • Su-Jae Lee
    • In-Chul Park
    • Young-Woo Jin
    • Sungkwan An
  • View Affiliations

  • Published online on: July 1, 2009     https://doi.org/10.3892/ijmm_00000204
  • Pages: 45-50
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Abstract

Suberoylanilide hydroxamic acid (SAHA) is a histone deacetylase inhibitor (HDACI) with antitumor effects that is being explored as a therapeutic drug. However, it has been reported that non-small cell lung cancer (NSCLC) is resistant to HDACIs. MicroRNAs (miRNAs) are a key class of small, non-coding RNA molecules that modulate post-transcriptional regulation of gene expression in multi-cellular organisms. miRNA expression patterns are involved in deregulation of gene expression in human lung cancer. Here we identified miRNA expression profile changes in response to SAHA treatment in the human lung carcinoma cell line A549. We also examined potential mRNA targets of SAHA-responsive miRNAs by using a target prediction program. Using microarray analysis, we found 64 miRNAs with >2-fold expression changes in SAHA-treated A549 cells. Among them, two unique miRNAs were altered in 2.5 µM SAHA-treated cells, 31 unique miRNAs were altered in 5.0 µM SAHA-treated cells and 31 miRNAs were altered with both doses. These miRNAs are predicted to have several target genes related to angiogenesis, apoptosis, chromatin modification, cell proliferation and differentiation. In conclusion, we have identified a unique set of miRNAs and their expression profiles that are influenced significantly by SAHA in the A549 NSCLC cell line model, which might provide useful information for understanding the anticancer mechanism of SAHA.

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July 2009
Volume 24 Issue 1

Print ISSN: 1107-3756
Online ISSN:1791-244X

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Spandidos Publications style
Lee E, Shin S, Cha HJ, Yoon Y, Bae S, Jung JH, Lee S, Lee S, Park I, Jin Y, Jin Y, et al: Suberoylanilide hydroxamic acid (SAHA) changes microRNA expression profiles in A549 human non-small cell lung cancer cells. Int J Mol Med 24: 45-50, 2009
APA
Lee, E., Shin, S., Cha, H.J., Yoon, Y., Bae, S., Jung, J.H. ... An, S. (2009). Suberoylanilide hydroxamic acid (SAHA) changes microRNA expression profiles in A549 human non-small cell lung cancer cells. International Journal of Molecular Medicine, 24, 45-50. https://doi.org/10.3892/ijmm_00000204
MLA
Lee, E., Shin, S., Cha, H. J., Yoon, Y., Bae, S., Jung, J. H., Lee, S., Lee, S., Park, I., Jin, Y., An, S."Suberoylanilide hydroxamic acid (SAHA) changes microRNA expression profiles in A549 human non-small cell lung cancer cells". International Journal of Molecular Medicine 24.1 (2009): 45-50.
Chicago
Lee, E., Shin, S., Cha, H. J., Yoon, Y., Bae, S., Jung, J. H., Lee, S., Lee, S., Park, I., Jin, Y., An, S."Suberoylanilide hydroxamic acid (SAHA) changes microRNA expression profiles in A549 human non-small cell lung cancer cells". International Journal of Molecular Medicine 24, no. 1 (2009): 45-50. https://doi.org/10.3892/ijmm_00000204