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International Journal of Molecular Medicine
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Print ISSN: 1107-3756 Online ISSN: 1791-244X
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August 2009 Volume 24 Issue 2

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International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

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Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

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Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

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Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

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International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

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International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

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Article

(−)-Epigallocatechin gallate attenuates the induction of HSP27 stimulated by sphingosine 1-phosphate via suppression of phosphatidylinositol 3-kinase/Akt pathway in osteoblasts

  • Authors:
    • Hideo Natsume
    • Seiji Adachi
    • Shinji Takai
    • Haruhiko Tokuda
    • Rie Matsushima-Nishiwaki
    • Chiho Minamitani
    • Junichi Yamauchi
    • Kanefusa Kato
    • Jun Mizutani
    • Osamu Kozawa
    • Takanobu Otsuka
  • View Affiliations / Copyright

    Affiliations: Department of Pharmacology, Gifu University Graduate School of Medicine, Gifu 501-1194, Japan
  • Pages: 197-203
    |
    Published online on: August 1, 2009
       https://doi.org/10.3892/ijmm_00000223
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Abstract

We previously reported that sphingosine 1-phosphate stimulates the induction of heat shock protein 27 (HSP27) through p38 mitogen-activated protein (MAP) kinase and phosphatidylinositol 3-kinase/Akt in osteoblast-like MC3T3-E1 cells. In the present study, we investigated whether (−)-epigallocatechin gallate (EGCG), the major polyphenol found in green tea, affects the induction of HSP27 in these cells and its mechanism, since it was previously reported that catechin, including EGCG, suppresses bone resorption. EGCG significantly reduced the induction of HSP27 stimulated by sphingosine 1-phosphate in a dose-dependent manner between 10 and 30 µM. Immunofluorescence microscopy studies revealed that sphingosine 1-phosphate certainly stimulated the induction of HSP27 in the cytosol of these cells, and that EGCG clearly suppressed its induction. However, sphingosine 1-phosphate-stimulated phosphorylation of p38 MAP kinase or MAPKAP-2 was not affected by EGCG. By contrast, EGCG markedly suppressed the phosphorylations of both Akt and glycogen synthase kinase-3β stimulated by sphingosine 1-phosphate. These results strongly suggest that EGCG suppresses the induction of HSP27 stimulated by sphingosine 1-phosphate via attenuation of, not the p38 MAP kinase pathway, but of the phosphatidylinositol 3-kinase/Akt pathway in osteoblasts.

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Copy and paste a formatted citation
Spandidos Publications style
Natsume H, Adachi S, Takai S, Tokuda H, Matsushima-Nishiwaki R, Minamitani C, Yamauchi J, Kato K, Mizutani J, Kozawa O, Kozawa O, et al: (−)-Epigallocatechin gallate attenuates the induction of HSP27 stimulated by sphingosine 1-phosphate via suppression of phosphatidylinositol 3-kinase/Akt pathway in osteoblasts. Int J Mol Med 24: 197-203, 2009.
APA
Natsume, H., Adachi, S., Takai, S., Tokuda, H., Matsushima-Nishiwaki, R., Minamitani, C. ... Otsuka, T. (2009). (−)-Epigallocatechin gallate attenuates the induction of HSP27 stimulated by sphingosine 1-phosphate via suppression of phosphatidylinositol 3-kinase/Akt pathway in osteoblasts. International Journal of Molecular Medicine, 24, 197-203. https://doi.org/10.3892/ijmm_00000223
MLA
Natsume, H., Adachi, S., Takai, S., Tokuda, H., Matsushima-Nishiwaki, R., Minamitani, C., Yamauchi, J., Kato, K., Mizutani, J., Kozawa, O., Otsuka, T."(−)-Epigallocatechin gallate attenuates the induction of HSP27 stimulated by sphingosine 1-phosphate via suppression of phosphatidylinositol 3-kinase/Akt pathway in osteoblasts". International Journal of Molecular Medicine 24.2 (2009): 197-203.
Chicago
Natsume, H., Adachi, S., Takai, S., Tokuda, H., Matsushima-Nishiwaki, R., Minamitani, C., Yamauchi, J., Kato, K., Mizutani, J., Kozawa, O., Otsuka, T."(−)-Epigallocatechin gallate attenuates the induction of HSP27 stimulated by sphingosine 1-phosphate via suppression of phosphatidylinositol 3-kinase/Akt pathway in osteoblasts". International Journal of Molecular Medicine 24, no. 2 (2009): 197-203. https://doi.org/10.3892/ijmm_00000223
Copy and paste a formatted citation
x
Spandidos Publications style
Natsume H, Adachi S, Takai S, Tokuda H, Matsushima-Nishiwaki R, Minamitani C, Yamauchi J, Kato K, Mizutani J, Kozawa O, Kozawa O, et al: (−)-Epigallocatechin gallate attenuates the induction of HSP27 stimulated by sphingosine 1-phosphate via suppression of phosphatidylinositol 3-kinase/Akt pathway in osteoblasts. Int J Mol Med 24: 197-203, 2009.
APA
Natsume, H., Adachi, S., Takai, S., Tokuda, H., Matsushima-Nishiwaki, R., Minamitani, C. ... Otsuka, T. (2009). (−)-Epigallocatechin gallate attenuates the induction of HSP27 stimulated by sphingosine 1-phosphate via suppression of phosphatidylinositol 3-kinase/Akt pathway in osteoblasts. International Journal of Molecular Medicine, 24, 197-203. https://doi.org/10.3892/ijmm_00000223
MLA
Natsume, H., Adachi, S., Takai, S., Tokuda, H., Matsushima-Nishiwaki, R., Minamitani, C., Yamauchi, J., Kato, K., Mizutani, J., Kozawa, O., Otsuka, T."(−)-Epigallocatechin gallate attenuates the induction of HSP27 stimulated by sphingosine 1-phosphate via suppression of phosphatidylinositol 3-kinase/Akt pathway in osteoblasts". International Journal of Molecular Medicine 24.2 (2009): 197-203.
Chicago
Natsume, H., Adachi, S., Takai, S., Tokuda, H., Matsushima-Nishiwaki, R., Minamitani, C., Yamauchi, J., Kato, K., Mizutani, J., Kozawa, O., Otsuka, T."(−)-Epigallocatechin gallate attenuates the induction of HSP27 stimulated by sphingosine 1-phosphate via suppression of phosphatidylinositol 3-kinase/Akt pathway in osteoblasts". International Journal of Molecular Medicine 24, no. 2 (2009): 197-203. https://doi.org/10.3892/ijmm_00000223
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