Molecular characterization and quantification using state of the art solid-state adiabatic TOBSY NMR in burn trauma

  • Authors:
    • Valeria Righi
    • Ovidiu Andronesi
    • Dionyssios Mintzopoulos
    • A. A. Tzika
  • View Affiliations

  • Published online on: December 1, 2009     https://doi.org/10.3892/ijmm_00000288
  • Pages: 749-757
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Abstract

We describe a novel solid-state nuclear magnetic resonance (NMR) method that maximizes the advantages of high-resolution magic-angle-spinning (HRMAS), relative conventional liquid-state NMR approaches, when applied to intact biopsies of skeletal muscle specimens collected from burn trauma patients. This novel method, termed optimized adiabatic TOtal through Bond correlation SpectroscopY (TOBSY) solid-state NMR pulse sequence for two-dimensional (2D) 1H-1H homonuclear scalar-coupling longitudinal isotropic mixing, was demonstrated to provide a 40-60% improvement in signal-to-noise ratio (SNR) relative to its liquid-state analogue TOCSY (TOtal Correlation SpectroscopY). Using 1- and 2-dimensional HRMAS NMR experiments, we identified several metabolites in burned tissues. Quantification of metabolites in burned tissues showed increased levels of lipid compounds, intracellular metabolites (e.g., taurine and phosphocreatine) and substantially decreased water-soluble metabolites (e.g., glutathione, carnosine, glucose, glutamine/ glutamate and alanine). These findings demonstrate that HRMAS NMR Spectroscopy using TOBSY is a feasible technique that reveals new insights into the pathophysiology of burn trauma. Moreover, this method has applications that facilitate the development of novel therapeutic strategies.

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December 2009
Volume 24 Issue 6

Print ISSN: 1107-3756
Online ISSN:1791-244X

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Spandidos Publications style
Righi V, Andronesi O, Mintzopoulos D and Tzika AA: Molecular characterization and quantification using state of the art solid-state adiabatic TOBSY NMR in burn trauma . Int J Mol Med 24: 749-757, 2009.
APA
Righi, V., Andronesi, O., Mintzopoulos, D., & Tzika, A.A. (2009). Molecular characterization and quantification using state of the art solid-state adiabatic TOBSY NMR in burn trauma . International Journal of Molecular Medicine, 24, 749-757. https://doi.org/10.3892/ijmm_00000288
MLA
Righi, V., Andronesi, O., Mintzopoulos, D., Tzika, A. A."Molecular characterization and quantification using state of the art solid-state adiabatic TOBSY NMR in burn trauma ". International Journal of Molecular Medicine 24.6 (2009): 749-757.
Chicago
Righi, V., Andronesi, O., Mintzopoulos, D., Tzika, A. A."Molecular characterization and quantification using state of the art solid-state adiabatic TOBSY NMR in burn trauma ". International Journal of Molecular Medicine 24, no. 6 (2009): 749-757. https://doi.org/10.3892/ijmm_00000288