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International Journal of Molecular Medicine
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Print ISSN: 1107-3756 Online ISSN: 1791-244X
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February 2010 Volume 25 Issue 2

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International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

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Article

Pancreatic acellular matrix supports islet survival and function in a synthetic tubular device: In vitro and in vivo studies

  • Authors:
    • E. De Carlo
    • S. Baiguera
    • M. T. Conconi
    • S. Vigolo
    • C. Grandi
    • S. Lora
    • C. Martini
    • P. Maffei
    • G. Tamagno
    • R. Vettor
    • N. Sicolo
    • P. P. Parnigotto
  • View Affiliations / Copyright

    Affiliations: Clinica Medica III, Hospital/University of Padova, I-35128 Padova, Italy. euge.decarlo@tiscali.it
  • Pages: 195-202
    |
    Published online on: February 1, 2010
       https://doi.org/10.3892/ijmm_00000330
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Abstract

Increasing pancreatic islet survival and function is a starting point for obtaining a valuable bioartificial pancreas for the treatment of type 1 diabetes. In this context, decellularized matrices, obtained after the removal of tissue cellular part, are known to support in vitro adhesion, growth, and function of several cell types. We demonstrate that a homologous acellular pancreatic matrix is a suitable scaffold for rat islet cultures maintaining their long-term viability and function. Islets adhered to the pancreatic matrix showed a constant glucose-induced insulin release during long-term in vitro incubation, while islets cultured without a matrix or on the liver matrix showed a progressive reduction. In order to obtain implantable devices, acellular matrix/islet cultures were entrapped into poly(vinyl alcohol) (PVA)/ poly(ethylene glycol) (PEG) tubes obtained by the freezing/thawing procedure. Under this condition, an in vitro constant insulin release was detected. The devices were then implanted into diabetic rats where reduced insulin requirement was noted suggesting insulin secretory activity of islets contained in the device. Indeed, immunofluorescence confirmed the presence of insulin- and glucagon-producing cells into the explanted devices. These data show that PVA/PEG semi-permeable membrane can obtain devices that restore, at least in part, insulin secretion.

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Copy and paste a formatted citation
Spandidos Publications style
De Carlo E, Baiguera S, Conconi MT, Vigolo S, Grandi C, Lora S, Martini C, Maffei P, Tamagno G, Vettor R, Vettor R, et al: Pancreatic acellular matrix supports islet survival and function in a synthetic tubular device: In vitro and in vivo studies. Int J Mol Med 25: 195-202, 2010.
APA
De Carlo, E., Baiguera, S., Conconi, M.T., Vigolo, S., Grandi, C., Lora, S. ... Parnigotto, P.P. (2010). Pancreatic acellular matrix supports islet survival and function in a synthetic tubular device: In vitro and in vivo studies. International Journal of Molecular Medicine, 25, 195-202. https://doi.org/10.3892/ijmm_00000330
MLA
De Carlo, E., Baiguera, S., Conconi, M. T., Vigolo, S., Grandi, C., Lora, S., Martini, C., Maffei, P., Tamagno, G., Vettor, R., Sicolo, N., Parnigotto, P. P."Pancreatic acellular matrix supports islet survival and function in a synthetic tubular device: In vitro and in vivo studies". International Journal of Molecular Medicine 25.2 (2010): 195-202.
Chicago
De Carlo, E., Baiguera, S., Conconi, M. T., Vigolo, S., Grandi, C., Lora, S., Martini, C., Maffei, P., Tamagno, G., Vettor, R., Sicolo, N., Parnigotto, P. P."Pancreatic acellular matrix supports islet survival and function in a synthetic tubular device: In vitro and in vivo studies". International Journal of Molecular Medicine 25, no. 2 (2010): 195-202. https://doi.org/10.3892/ijmm_00000330
Copy and paste a formatted citation
x
Spandidos Publications style
De Carlo E, Baiguera S, Conconi MT, Vigolo S, Grandi C, Lora S, Martini C, Maffei P, Tamagno G, Vettor R, Vettor R, et al: Pancreatic acellular matrix supports islet survival and function in a synthetic tubular device: In vitro and in vivo studies. Int J Mol Med 25: 195-202, 2010.
APA
De Carlo, E., Baiguera, S., Conconi, M.T., Vigolo, S., Grandi, C., Lora, S. ... Parnigotto, P.P. (2010). Pancreatic acellular matrix supports islet survival and function in a synthetic tubular device: In vitro and in vivo studies. International Journal of Molecular Medicine, 25, 195-202. https://doi.org/10.3892/ijmm_00000330
MLA
De Carlo, E., Baiguera, S., Conconi, M. T., Vigolo, S., Grandi, C., Lora, S., Martini, C., Maffei, P., Tamagno, G., Vettor, R., Sicolo, N., Parnigotto, P. P."Pancreatic acellular matrix supports islet survival and function in a synthetic tubular device: In vitro and in vivo studies". International Journal of Molecular Medicine 25.2 (2010): 195-202.
Chicago
De Carlo, E., Baiguera, S., Conconi, M. T., Vigolo, S., Grandi, C., Lora, S., Martini, C., Maffei, P., Tamagno, G., Vettor, R., Sicolo, N., Parnigotto, P. P."Pancreatic acellular matrix supports islet survival and function in a synthetic tubular device: In vitro and in vivo studies". International Journal of Molecular Medicine 25, no. 2 (2010): 195-202. https://doi.org/10.3892/ijmm_00000330
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