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International Journal of Molecular Medicine
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Print ISSN: 1107-3756 Online ISSN: 1791-244X
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February 2010 Volume 25 Issue 2

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International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

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Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

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Article

Degradation and destabilization of abnormal prion protein using alkaline detergents and proteases

  • Authors:
    • Yoshihiko Hirata
    • Hitoshi Ito
    • Taro Furuta
    • Kazuyoshi Ikuta
    • Akikazu Sakudo

  • View Affiliations / Copyright

    Affiliations: Biochemical Laboratory, Saraya Co., Ltd., 24-12, Tamate-cho, Kashiwara, Osaka 582-0028, Japan
  • Pages: 267-270
    |
    Published online on: February 1, 2010
       https://doi.org/10.3892/ijmm_00000340
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Abstract

There is a limited number of reports regarding detergents and proteases inactivating, degrading, or destabilizing abnormal prion protein (PrPSc). In the present study, the effect of alkaline detergents and proteases on the breakdown of PrPSc in the absence of proteinase K (PK) (degradation) and the presence of PK (destabilization) was investigated. PrPSc from brain homogenate of terminally-diseased mice infected with the Chandler strain of scrapie was used as a substrate. A surfactant-free alkaline detergent (pH 11.9, 1% aqueous solution) with potassium hydroxide as the main ingredient and an alkaline detergent (pH 11.9, 1% aqueous solution) containing about 1% surfactant as well as two commercially available alkaline proteases had a destabilizing effect on PrPSc. All these detergents and proteases showed degradative effects on PrPSc under appropriate conditions. These results demonstrate the usefulness of alkaline detergents and proteases for the degradation or destabilization of PrPSc.

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Copy and paste a formatted citation
Spandidos Publications style
Hirata Y, Ito H, Furuta T, Ikuta K and Sakudo A: Degradation and destabilization of abnormal prion protein using alkaline detergents and proteases. Int J Mol Med 25: 267-270, 2010.
APA
Hirata, Y., Ito, H., Furuta, T., Ikuta, K., & Sakudo, A. (2010). Degradation and destabilization of abnormal prion protein using alkaline detergents and proteases. International Journal of Molecular Medicine, 25, 267-270. https://doi.org/10.3892/ijmm_00000340
MLA
Hirata, Y., Ito, H., Furuta, T., Ikuta, K., Sakudo, A."Degradation and destabilization of abnormal prion protein using alkaline detergents and proteases". International Journal of Molecular Medicine 25.2 (2010): 267-270.
Chicago
Hirata, Y., Ito, H., Furuta, T., Ikuta, K., Sakudo, A."Degradation and destabilization of abnormal prion protein using alkaline detergents and proteases". International Journal of Molecular Medicine 25, no. 2 (2010): 267-270. https://doi.org/10.3892/ijmm_00000340
Copy and paste a formatted citation
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Spandidos Publications style
Hirata Y, Ito H, Furuta T, Ikuta K and Sakudo A: Degradation and destabilization of abnormal prion protein using alkaline detergents and proteases. Int J Mol Med 25: 267-270, 2010.
APA
Hirata, Y., Ito, H., Furuta, T., Ikuta, K., & Sakudo, A. (2010). Degradation and destabilization of abnormal prion protein using alkaline detergents and proteases. International Journal of Molecular Medicine, 25, 267-270. https://doi.org/10.3892/ijmm_00000340
MLA
Hirata, Y., Ito, H., Furuta, T., Ikuta, K., Sakudo, A."Degradation and destabilization of abnormal prion protein using alkaline detergents and proteases". International Journal of Molecular Medicine 25.2 (2010): 267-270.
Chicago
Hirata, Y., Ito, H., Furuta, T., Ikuta, K., Sakudo, A."Degradation and destabilization of abnormal prion protein using alkaline detergents and proteases". International Journal of Molecular Medicine 25, no. 2 (2010): 267-270. https://doi.org/10.3892/ijmm_00000340
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