A cell-microelectronic sensing technique for the screening of cytoprotective compounds

  • Authors:
    • Béla Ózsvári
    • László G. Puskás
    • Lajos I. Nagy
    • Iván Kanizsai
    • Márió Gyuris
    • Ramóna Madácsi
    • Liliána Z. Fehér
    • Domokos Gerö
    • Csaba Szabó
  • View Affiliations

  • Published online on: April 1, 2010     https://doi.org/10.3892/ijmm_00000373
  • Pages: 525-530
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Abstract

In recent years, a new cell-based high throughput paradigm has emerged, which seeks to identify novel, pharmacologically active cytoprotective compounds. The essence of this approach is to create experimental models of cell injury relevant for a particular disease by establishing in vitro cell-based models, followed by high-throughput testing of compounds that affect the cellular response in a desired manner. Prior approaches typically used simple end-point analyses. To assess the cytoprotective effects of novel drug candidates in real-time, we have applied a cell-microelectronic sensing technique (RT-CES), which measures changes in the impedance of individual microelectronic wells that correlates linearly with cell index (reflecting cell number, adherence and cell growth), thereby allowing the continuous determination of cell viability during oxidative stress. In vitro cytotoxicity was elicited by hydrogen peroxide in myocytes (H9c2) and hepatocytes (Hep3B). Cells were post-treated at 30 min with various reference molecules and novel cytoprotective compounds. Cytoprotection detected in the RT-CES system correlated well with the results of two classical end-point-based methods (improvement in MTT and reduction of LDH release). The RT-CES method, when used as described in the current report, is suitable for the screening of molecular libraries to identify molecules or molecule combinations that attenuate oxidative stress-induced cell damage.

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April 2010
Volume 25 Issue 4

Print ISSN: 1107-3756
Online ISSN:1791-244X

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Spandidos Publications style
Ózsvári B, Puskás LG, Nagy LI, Kanizsai I, Gyuris M, Madácsi R, Fehér LZ, Gerö D and Szabó C: A cell-microelectronic sensing technique for the screening of cytoprotective compounds. Int J Mol Med 25: 525-530, 2010.
APA
Ózsvári, B., Puskás, L.G., Nagy, L.I., Kanizsai, I., Gyuris, M., Madácsi, R. ... Szabó, C. (2010). A cell-microelectronic sensing technique for the screening of cytoprotective compounds. International Journal of Molecular Medicine, 25, 525-530. https://doi.org/10.3892/ijmm_00000373
MLA
Ózsvári, B., Puskás, L. G., Nagy, L. I., Kanizsai, I., Gyuris, M., Madácsi, R., Fehér, L. Z., Gerö, D., Szabó, C."A cell-microelectronic sensing technique for the screening of cytoprotective compounds". International Journal of Molecular Medicine 25.4 (2010): 525-530.
Chicago
Ózsvári, B., Puskás, L. G., Nagy, L. I., Kanizsai, I., Gyuris, M., Madácsi, R., Fehér, L. Z., Gerö, D., Szabó, C."A cell-microelectronic sensing technique for the screening of cytoprotective compounds". International Journal of Molecular Medicine 25, no. 4 (2010): 525-530. https://doi.org/10.3892/ijmm_00000373