The effects of N-acetyl cysteine on the MG132 proteasome inhibitor-treated lung cancer cells in relation to cell growth, reactive oxygen species and glutathione

  • Authors:
    • Yong Hwan Han
    • Hwa Jin Moon
    • Bo Ra You
    • Sung Zoo Kim
    • Suhn Hee Kim
    • Woo Hyun Park
  • View Affiliations

  • Published online on: April 1, 2010     https://doi.org/10.3892/ijmm_00000389
  • Pages: 657-662
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Abstract

MG132 as a proteasome inhibitor has been shown to induce apoptotic cell death through formation of reactive oxygen species (ROS). Here, we investigated the effects of N-acetyl cysteine (NAC; a well-known antioxidant), L-buthionine sulfoximine (BSO; an inhibitor of GSH synthesis) or diethyldithiocarbamate (DDC; an inhibitor of Cu/Zn-SOD) on MG132-treated Calu-6 or A549 lung cancer cells in relation to cell growth, ROS and GSH levels. MG132 inhibited the growth of Calu-6 and A549 cells at 24 h. MG132 induced apoptosis in both cell lines, which was accompanied by the loss of mitochondrial membrane potential (MMP; ΔΨm). ROS levels including O2·− were increased in both MG132-treated lung cells. MG132 also induced GSH depletion in both lung cell types. Treatment with 10 µM BSO or 1 µM DDC affected ROS and GSH levels in MG132-treated Calu-6 cells. However, these changes did not influence cell growth and death in the cells. NAC prevented cell growth inhibition and death in MG132-treated lung cells, which was accompanied by decreased ROS, but not by decreased GSH depletion. In conclusion, the changes of ROS and GSH by MG132, NAC, BSO or DDC were partially related to cell growth and death in the lung cancer cell lines Calu-6 and A549.

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April 2010
Volume 25 Issue 4

Print ISSN: 1107-3756
Online ISSN:1791-244X

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Spandidos Publications style
Han YH, Moon HJ, You BR, Kim SZ, Kim SH and Park WH: The effects of N-acetyl cysteine on the MG132 proteasome inhibitor-treated lung cancer cells in relation to cell growth, reactive oxygen species and glutathione. Int J Mol Med 25: 657-662, 2010.
APA
Han, Y.H., Moon, H.J., You, B.R., Kim, S.Z., Kim, S.H., & Park, W.H. (2010). The effects of N-acetyl cysteine on the MG132 proteasome inhibitor-treated lung cancer cells in relation to cell growth, reactive oxygen species and glutathione. International Journal of Molecular Medicine, 25, 657-662. https://doi.org/10.3892/ijmm_00000389
MLA
Han, Y. H., Moon, H. J., You, B. R., Kim, S. Z., Kim, S. H., Park, W. H."The effects of N-acetyl cysteine on the MG132 proteasome inhibitor-treated lung cancer cells in relation to cell growth, reactive oxygen species and glutathione". International Journal of Molecular Medicine 25.4 (2010): 657-662.
Chicago
Han, Y. H., Moon, H. J., You, B. R., Kim, S. Z., Kim, S. H., Park, W. H."The effects of N-acetyl cysteine on the MG132 proteasome inhibitor-treated lung cancer cells in relation to cell growth, reactive oxygen species and glutathione". International Journal of Molecular Medicine 25, no. 4 (2010): 657-662. https://doi.org/10.3892/ijmm_00000389