CLONAL EVOLUTION AND TUMOR PROGRESSION IN 2 HUMAN COLORECTAL ADENOMA-DERIVED CELL-LINES INVITRO - THE INVOLVEMENT OF CHROMOSOME-1 ABNORMALITIES

  • Authors:
    • A HAGUE
    • KA HANLON
    • C PARASKEVA
  • View Affiliations

  • Published online on: July 1, 1992     https://doi.org/10.3892/ijo.1.2.201
  • Pages: 201-208
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Abstract

Two human colorectal adenoma cell lines, S/RG and S/AN, have been continuously passaged in vitro to determine whether they would immortalize and if specific cytogenetic changes were involved in immortalization and tumor progression. At passage 7, S/RG was highly aneuploid, but had no abnormalities of chromosome 1 (Paraskeva et al, Cancer Res 49: 1282-1286, 1989). With continued passage under two independent sets of growth conditions an isochromosome Iq and derivatives of this isochromosome occurred as specific abnormalities. S/AN was near-diploid at passage 10, with a deletion in lp and monosomy 18. The karyotype at passage 44 showed no change. The cell lines are stable in that they have remained anchorage-dependent and non-tumorigenic after several years in culture and S/AN has retained a near diploid karyotype. These cell lines are therefore highly valuable for further studies of tumor progression in human colorectal carcinogenesis.

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July 1992
Volume 1 Issue 2

Print ISSN: 1019-6439
Online ISSN:1791-2423

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Spandidos Publications style
HAGUE A, HANLON K and PARASKEVA C: CLONAL EVOLUTION AND TUMOR PROGRESSION IN 2 HUMAN COLORECTAL ADENOMA-DERIVED CELL-LINES INVITRO - THE INVOLVEMENT OF CHROMOSOME-1 ABNORMALITIES. Int J Oncol 1: 201-208, 1992
APA
HAGUE, A., HANLON, K., & PARASKEVA, C. (1992). CLONAL EVOLUTION AND TUMOR PROGRESSION IN 2 HUMAN COLORECTAL ADENOMA-DERIVED CELL-LINES INVITRO - THE INVOLVEMENT OF CHROMOSOME-1 ABNORMALITIES. International Journal of Oncology, 1, 201-208. https://doi.org/10.3892/ijo.1.2.201
MLA
HAGUE, A., HANLON, K., PARASKEVA, C."CLONAL EVOLUTION AND TUMOR PROGRESSION IN 2 HUMAN COLORECTAL ADENOMA-DERIVED CELL-LINES INVITRO - THE INVOLVEMENT OF CHROMOSOME-1 ABNORMALITIES". International Journal of Oncology 1.2 (1992): 201-208.
Chicago
HAGUE, A., HANLON, K., PARASKEVA, C."CLONAL EVOLUTION AND TUMOR PROGRESSION IN 2 HUMAN COLORECTAL ADENOMA-DERIVED CELL-LINES INVITRO - THE INVOLVEMENT OF CHROMOSOME-1 ABNORMALITIES". International Journal of Oncology 1, no. 2 (1992): 201-208. https://doi.org/10.3892/ijo.1.2.201