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International Journal of Oncology
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Print ISSN: 1019-6439 Online ISSN: 1791-2423
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October 1992 Volume 1 Issue 5

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Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

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October 1992 Volume 1 Issue 5

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Article

EXPRESSION OF INDIVIDUAL RAS PROTEINS IN NORMAL AND NEOPLASTIC COLON

  • Authors:
    • S PORIES
    • P GREATHEAD
    • G STEELE
    • IC SUMMERHAYES
  • View Affiliations / Copyright

    Affiliations: HARVARD UNIV,NEW ENGLAND DEACONESS HOSP,SCH MED,DEPT SURG,CANC BIOL LAB,50 BINNEY ST,BOSTON,MA 02215.
  • Pages: 581-585
    |
    Published online on: October 1, 1992
       https://doi.org/10.3892/ijo.1.5.581
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Abstract

In this study, we report the use of a panel of four ras antibodies in Western blot analysis which characterize the expression of individual ras members in different cell populations. The specificity of the different antibodies to H-, N- or K-ras proteins was established in a panel of NIH/3T3 transfectants and human cell lines harboring known ras oncogenic events. Localization of individual ras members in one dimensional gel electrophoresis revealed slower migration of K-ras p21 products, whilst H- and N-ras proteins co-migrated in this system. Identification of migrational differences between activated proto-oncogene products was facilitated using ras member specific probes. Application of this approach to normal gastrointestinal mucosa.revealed over-representation of K-ras p21 in both stomach and colon epithelium in the rat. This differential ras profile was maintained in normal human colon mucosa and colon tumors from the same individual. Use of such antibodies now enables dissection of ras proto-oncoprotein expression in different tissues and compartments within tissue with the ability to characterize ras mutational events in neoplastic progression.

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Copy and paste a formatted citation
Spandidos Publications style
PORIES S, GREATHEAD P, STEELE G and SUMMERHAYES I: EXPRESSION OF INDIVIDUAL RAS PROTEINS IN NORMAL AND NEOPLASTIC COLON. Int J Oncol 1: 581-585, 1992.
APA
PORIES, S., GREATHEAD, P., STEELE, G., & SUMMERHAYES, I. (1992). EXPRESSION OF INDIVIDUAL RAS PROTEINS IN NORMAL AND NEOPLASTIC COLON. International Journal of Oncology, 1, 581-585. https://doi.org/10.3892/ijo.1.5.581
MLA
PORIES, S., GREATHEAD, P., STEELE, G., SUMMERHAYES, I."EXPRESSION OF INDIVIDUAL RAS PROTEINS IN NORMAL AND NEOPLASTIC COLON". International Journal of Oncology 1.5 (1992): 581-585.
Chicago
PORIES, S., GREATHEAD, P., STEELE, G., SUMMERHAYES, I."EXPRESSION OF INDIVIDUAL RAS PROTEINS IN NORMAL AND NEOPLASTIC COLON". International Journal of Oncology 1, no. 5 (1992): 581-585. https://doi.org/10.3892/ijo.1.5.581
Copy and paste a formatted citation
x
Spandidos Publications style
PORIES S, GREATHEAD P, STEELE G and SUMMERHAYES I: EXPRESSION OF INDIVIDUAL RAS PROTEINS IN NORMAL AND NEOPLASTIC COLON. Int J Oncol 1: 581-585, 1992.
APA
PORIES, S., GREATHEAD, P., STEELE, G., & SUMMERHAYES, I. (1992). EXPRESSION OF INDIVIDUAL RAS PROTEINS IN NORMAL AND NEOPLASTIC COLON. International Journal of Oncology, 1, 581-585. https://doi.org/10.3892/ijo.1.5.581
MLA
PORIES, S., GREATHEAD, P., STEELE, G., SUMMERHAYES, I."EXPRESSION OF INDIVIDUAL RAS PROTEINS IN NORMAL AND NEOPLASTIC COLON". International Journal of Oncology 1.5 (1992): 581-585.
Chicago
PORIES, S., GREATHEAD, P., STEELE, G., SUMMERHAYES, I."EXPRESSION OF INDIVIDUAL RAS PROTEINS IN NORMAL AND NEOPLASTIC COLON". International Journal of Oncology 1, no. 5 (1992): 581-585. https://doi.org/10.3892/ijo.1.5.581
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