Structural specificity and tumoricidal action of methyl-3,5-diiodo-4-(4'-methoxyphenoxy) benzoate (DIME)

  • Authors:
    • J Mendeleyev
    • L Young
    • E Kirsten
    • C Vidair
    • A Hakam
    • J Zhen
    • P Bauer
    • K Buki
    • E Kun
  • View Affiliations

  • Published online on: April 1, 1997     https://doi.org/10.3892/ijo.10.4.689
  • Pages: 689-695
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Abstract

Seventeen homologs and analogs of methyl-3,5-diiodo-4(4'-methoxyphenoxy) benzoate (DIME), a hormonally inactive analog of thyroid hormones, have been synthesized and their antitumor activity scored by assaying their antitumorigenic effect in vivo following pretreatment of E-ras 20 cells with the drug. In vivo feeding of DIME in large doses had a similar antitumor effect on human tumor xenogafts in vivo without noticeable toxicity of DIME. Inhibition of clonogenicity with MDA-MB-231 cells by DIME yielded I-50 values similar to those found in tests measuring cell growth inhibition (median I-50 less than 1.0 mu M) The apparent ultimate signal for cytocidal action of DIME is the development of dose-dependent double strand cuts of DNA. All human tumor cells so far tested, with the exception of A-549 cells (lung cancer), show high sensitivity to DIME (I-50 1.0 mu M or below). The partially refractory behavior of A-549 cells to DIME is due to the presence of an esterase that cleaves the methyl ester group of DIME. Other tumor cells have negligible esterase activity, while such activity in homogenates of normal mouse tissues is high. Cells in culture take up DIME and the magnitude of uptake parallels drug sensitivity to DIME. Extrapolation from the correlations between drug efficacy, drug uptake, esterase activity and the absence of significant in vivo toxicity of DIME point to uptake of DIME by cells in culture but not by normal cells operating in intact organs in vivo. In contrast, tumor cells in vivo take up DIME and succumb to its cytocidal action just like cells in culture.

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April 1997
Volume 10 Issue 4

Print ISSN: 1019-6439
Online ISSN:1791-2423

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Spandidos Publications style
Mendeleyev J, Young L, Kirsten E, Vidair C, Hakam A, Zhen J, Bauer P, Buki K and Kun E: Structural specificity and tumoricidal action of methyl-3,5-diiodo-4-(4'-methoxyphenoxy) benzoate (DIME). Int J Oncol 10: 689-695, 1997
APA
Mendeleyev, J., Young, L., Kirsten, E., Vidair, C., Hakam, A., Zhen, J. ... Kun, E. (1997). Structural specificity and tumoricidal action of methyl-3,5-diiodo-4-(4'-methoxyphenoxy) benzoate (DIME). International Journal of Oncology, 10, 689-695. https://doi.org/10.3892/ijo.10.4.689
MLA
Mendeleyev, J., Young, L., Kirsten, E., Vidair, C., Hakam, A., Zhen, J., Bauer, P., Buki, K., Kun, E."Structural specificity and tumoricidal action of methyl-3,5-diiodo-4-(4'-methoxyphenoxy) benzoate (DIME)". International Journal of Oncology 10.4 (1997): 689-695.
Chicago
Mendeleyev, J., Young, L., Kirsten, E., Vidair, C., Hakam, A., Zhen, J., Bauer, P., Buki, K., Kun, E."Structural specificity and tumoricidal action of methyl-3,5-diiodo-4-(4'-methoxyphenoxy) benzoate (DIME)". International Journal of Oncology 10, no. 4 (1997): 689-695. https://doi.org/10.3892/ijo.10.4.689